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VERTEBRAL DEVELOPMENT AND PROTEASE ACTIVITY OF LARVAL MILKFISH, Chanos chanos Forsskal IN DIFFERENT REARING MEDIA Titiek Aslianti; Afifah Nasukha; Irwan Setyadi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.266 KB) | DOI: 10.29244/jitkt.v6i1.8630

Abstract

The milkfish (Chanos chanos Forsskal) seed quality is an important factor for supporting the fisheries industrialization program . The egg fertility and larval rearing management are two main factors to improve the quality of the seed. The aim of this research was to obtain the information about those two indicators on milkfish larvae, reared in different media. About 100,000 eggs were located in 6 units of the outdoor concrete tank with volume of 6 m3. Larvae were reared due to the rearing standard procedure while the feeding management was adapted during larvae development. Two different treatments in larval rearing medium were with and without the addition of molasses, subsequently in triple trials. This study started from hatching day (0 DAH) until the end of larval rearing period (16-18 DAH). The growth parameters such as vertebrae analyses and survival were taken in the end of research periods, while protease assessment was analyzed every day using tyrosine and casein standard methods. The results showed  that  the molasses addition produced a higher growth (TL 12.95±0.87 mm) and survival rate (87.66±1.52%), better vertebral performance (44-45 segments), and higher protease activity (0.1392-0.3863 U/ml/min) than without molasses addition. The results indicated that molasses addition in larvae rearing medium improved the larvae quality. Keywords: Protease enzime activity,milkfish seeds, molasses, vertebrae
NATURAL FEED (ROTIFER) MANAGEMENT ON ORANGE SPOTTED GROUPER Epinephelus coioides LARVAL REARING Titiek Aslianti; Irwan Setyadi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 2 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.758 KB) | DOI: 10.29244/jitkt.v6i2.9015

Abstract

ABSTRACT According to Indonesian Standard Quality, the optimal seed production of orange spotted grouper (Epinephelus coioides) has been unsuccessful due to the problems occurred during larval rearing period. Efforts on mass rearing of larvae has been carried out in this study to optimize the natural feeding (rotifer). The purpose of this research was to obtain the information about an appropriate duration time for natural feeding (rotifer) to produce higher survival rate. The treatment for providing rotifers on larvae (days after hatching/DAH) i.e., (A) 20 days, (B) 25 days, (C) 30 days, and (D) 35 days. Rotifers were enriched with DHA Selco. Experiment used fiber tanks with 1 m3 capacity with a completely randomized design (CRD) and three replicates. The larvae was reared to achieve the juvenile phase (D-45). The best results from 1 m3 tanks were then reared using concrete tanks of 6 m3 and 10 m3 capacities. The results showed that feeding of rotifers up to 25 DAH larvae on fiber tanks (1 m3) produced the hughest survival rate (7.63 %). Meanwhile, the mass reared in 6 m3 and 10 m3 concrete tanks produced  survival rate of 4.9±3,54% and 8.8±2.26%, respectively. The homogenous size was relatively high (80%) and the seed abnormality was relatively low (0.4%).   Keywords: rotifers, feeding time, Orange Spotted  grouper larvae, Epinephelus coioides