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The dry matter digestibility characteristics of tropical tree legumes using menke in vitro gas production technique Pamungkas, Dicky; Sevilla, Cesar C
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 4 (2003)
Publisher : Indonesian Animal Sciences Society

The amount of gas released when a feed was incubated in vitro with rumen fluid, was closely related to the digestibility of the feed. The experiment using Menke in vitro gas production was conducted to determine the dry matter digestibility of tropical tree legumes; Leucaena leucocephala, Flemingia macrophyla, and Gliricida sepium. The samples were collected from Institute of Animal Science farms of the University of the Philippines at Los Banos. The amount of gas produced were recorded at 3, 6, 12, 48 and 72 hours of incubation. To estimate the volume of gas, the equation of Tuah was adopted; while to describe the course of gas production, the equation of MCDONALD was used. The washing loss was not measured and assumed to be zero. Results showed that leucaena at 12 hours was the highest in the volume of gas produced (1.217 ml/g DM). There was a similarity of highly increase of the gas produced between gliricidia and flemingia at 24 hours of incubation i.e 1.60 ml/g DM and 1.60ml/g DM. The potential extent gas production at 72 hours (b value) and the gas production rate (c value) of three legumes were no significantly different. According to the logarithmic-curve of gas volume produced, leucaena tended to be the highest of increase in potential extent and the gas production rate. The different rate of gas production in related to incubation reflected the dry matter digestibility of the three legumes in the rumen. Â Key words: In vitro gas production, dry matter digestibility

SEVILLA and U.M. LUSTRIA. 2006. Changes in rumen ecosystem and feed dry matter degradability of buffalo which received rumen content of cattle through cross inoculation Pamungkas, Dicky; Sevilla, Cesar C; Lustria, Ulysses M
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 1 (2006)
Publisher : Indonesian Animal Sciences Society

The research was done to identify changes in rumen ecosystem of buffalo which received rumen content of cattle. As much as three head of fistulated male buffaloes (live weight of 450-550 kg) and three fistulated female cattle (live weight 250-380 kg) were used. This experiment was done three stage as follows: pre-inoculation, inoculation and post-inoculation. In Pre-inoculation, the sample of rumen content was taken two hours before morning feeding and directly observed for pH rumen liquor, ammonia nitrogen (NH3-N) and total volatile fatty acid (VFA). During the last three days of the first week of pre and post inoculation, the in situ dry matter digestibility was conducted. The samples were incubated for 0, 3, 6, 9, 12, 24, 48, and 72 hours in the rumen of the experimental animals. Napier grass, leucaena, and wheat pollard were incubated separately in the nylon bags to determine DM digestibility. The cross inoculation (total of rumen content) was done less than 10 minutes. In post-inoculation, the sample of rumen content was taken at two hours before morning feeding as long as two days of two weeks after inoculation. Changes in rumen ecosystem (pH, NH3-N and total VFA) were tested by using Completely Randomized Design. Result showed that the transfer of rumen content from cattle to buffalo significantly reduced the pH level in the rumen of buffalo. However, it had no significant effect on the ammonia concentration in the rumen of buffalo and showed significantly decreased of bacteria but it was not affect on the total count of protozoa and fungi. The transfer of rumen content from cattle to buffalo significantly decreased the value of insoluble and potential digestible fraction of leucaena. The DM effective degradability of Napier grass was significantly increase (P<0.05) within outflow rate 0.02 and 0.04 h-1 after cross inoculation. In wheat pollard, a significantly decrease (P<0.05) was occurred when outflow rate was observed at 0.04, 0.06 and 0.08 h-1 Key Words: Rumen Ecosystem, Cross Inoculation, Degradability

The dry matter digestibility characteristics of tropical tree legumes using menke in vitro gas production technique Dicky Pamungkas; Cesar C Sevilla
Jurnal Ilmu Ternak dan Veteriner Vol 8, No 4 (2003): DECEMBER 2003
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The amount of gas released when a feed was incubated in vitro with rumen fluid, was closely related to the digestibility of the feed. The experiment using Menke in vitro gas production was conducted to determine the dry matter digestibility of tropical tree legumes; Leucaena leucocephala, Flemingia macrophyla, and Gliricida sepium. The samples were collected from Institute of Animal Science farms of the University of the Philippines at Los Banos. The amount of gas produced were recorded at 3, 6, 12, 48 and 72 hours of incubation. To estimate the volume of gas, the equation of Tuah was adopted; while to describe the course of gas production, the equation of MCDONALD was used. The washing loss was not measured and assumed to be zero. Results showed that leucaena at 12 hours was the highest in the volume of gas produced (1.217 ml/g DM). There was a similarity of highly increase of the gas produced between gliricidia and flemingia at 24 hours of incubation i.e 1.60 ml/g DM and 1.60ml/g DM. The potential extent gas production at 72 hours (b value) and the gas production rate (c value) of three legumes were no significantly different. According to the logarithmic-curve of gas volume produced, leucaena tended to be the highest of increase in potential extent and the gas production rate. The different rate of gas production in related to incubation reflected the dry matter digestibility of the three legumes in the rumen. Key words: In vitro gas production, dry matter digestibility

Changes in rumen ecosystem and feed dry matter degradability of buffalo which received rumen content of cattle through cross inoculation Dicky Pamungkas; Cesar C Sevilla; Ulysses M Lustria
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 1 (2006): MARCH 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The research was done to identify changes in rumen ecosystem of buffalo which received rumen content of cattle. As much as three head of fistulated male buffaloes (live weight of 450-550 kg) and three fistulated female cattle (live weight 250-380 kg) were used. This experiment was done three stage as follows: pre-inoculation, inoculation and post-inoculation. In Pre-inoculation, the sample of rumen content was taken two hours before morning feeding and directly observed for pH rumen liquor, ammonia nitrogen (NH3-N) and total volatile fatty acid (VFA). During the last three days of the first week of pre and post inoculation, the in situ dry matter digestibility was conducted. The samples were incubated for 0, 3, 6, 9, 12, 24, 48, and 72 hours in the rumen of the experimental animals. Napier grass, leucaena, and wheat pollard were incubated separately in the nylon bags to determine DM digestibility. The cross inoculation (total of rumen content) was done less than 10 minutes. In post-inoculation, the sample of rumen content was taken at two hours before morning feeding as long as two days of two weeks after inoculation. Changes in rumen ecosystem (pH, NH3-N and total VFA) were tested by using Completely Randomized Design. Result showed that the transfer of rumen content from cattle to buffalo significantly reduced the pH level in the rumen of buffalo. However, it had no significant effect on the ammonia concentration in the rumen of buffalo and showed significantly decreased of bacteria but it was not affect on the total count of protozoa and fungi. The transfer of rumen content from cattle to buffalo significantly decreased the value of insoluble and potential digestible fraction of leucaena. The DM effective degradability of Napier grass was significantly increase (P<0.05) within outflow rate 0.02 and 0.04 h-1 after cross inoculation. In wheat pollard, a significantly decrease (P<0.05) was occurred when outflow rate was observed at 0.04, 0.06 and 0.08 h-1Key Words: Rumen Ecosystem, Cross Inoculation, Degradability

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