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P. Singh
Department of Biochemistry Nepalgunj medical college, Chisapani Banke , Nepal
Medicine & Pharmacology

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PREVALENCE OF ANTI-STREPTOLYSIN O ANTIBODIES AT BANKE REGION NEPAL Khan, S.; Singh, P.; Siddiqui, A.H.
BALI MEDICAL JOURNAL Volume 1, Number 3, September-December 2012
Publisher : BALI MEDICAL JOURNAL

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Objective: Serum levels of anti-streptolysin O antibodies (ASO) in the routine evaluation of patients with rheumatic conditions. Prevalence of elevated serum ASO titer in patients which were coming in OPD & IPD of Nepalgunj Medical College & teaching hospital with various clinical conditions. Method: This was a retrospective cross sectional study, which was performed in the central laboratory of Microbiology at Nepalgunj Medical College and teaching Hospital, Banke, Nepal during the period of September 2010 to April 2012. The serum samples were tested for Antistreptolysin O (ASO) antibodies by latex agglutination test. Results: Total 308 patients including 40.91% male and 50.09% female  were tested for ASO serum levels.140 were positive and 168 were negative .In 140 positive cases 57 were male and 83 were female. In 168 negative cases 69 were male and 99 were female. Conclusion: The prevalence of Anti-streptolysin O (ASO) antibody in total cases was 45.45%.In male was 45.24% and in female was 45.60%.the highest percentage was found in age group 0-21years. The presence of elevated streptococcal antibody titers in such a population, which probably re?ects a high background prevalence of streptococcal infections, should be taken into consideration when evaluating the role of the group A streptococcus in non-purulent complications of infections.
PREVALENCE OF HYPERURICEMIA AT NEPALGUNJ MEDICAL COLLEGE, BANKE-NEPAL Singh, P.; Khan, S.; Mittal, R.K.
BALI MEDICAL JOURNAL Volume 1, Number 3, September-December 2012
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Objective: The prevalence of hyperuricemia varied in different populations and it appeared to be increasing in the past decades. Recent studies suggest that hyperuricemia is an independent risk factor for cardiovascular disease. Method: One thousand eighty seven patients, attending Nepalgunj medical college and Teaching Hospital, Banke, Nepal from March 2011 to February 2012 were included in this study. The reference range was 3.5 – 7.0 mg/dL in males and 2.6 – 6.0 mg/dL in females. Hyperuricemia was de?ned as a Serum Uric Acid level of more than 7.0 mg/dL in males and of more than 6.0 mg/dL in females. The uric acid was determined by uricase /PAP method. Test was performed in the central laboratory of Biochemistry, Nepalgunj Medical College & teaching Hospital, Banke, Nepal. Results: A number of 1487 studies were selected, the statistical information of which was collected for systematic analysis. The results showed that the high prevalence of hyperuricemia found in females(22.86%) as compare to males(18.98). It was found that 21-40 age group is on high risk for hyperuricemia.Conclusion: The prevalence of hyperuricemia is different as the period of age and it increases after 21-40 years in male and in female. Serum uric acid level was high in female as compare to male.
PREVALENCE OF JAUNDICE BASED ON LIVER FUNCTION TEST IN WESTERN NEPAL Singh, P.; Khan, S.; Mittal, and R. K.
BALI MEDICAL JOURNAL Volume 2 Number 2, May-August 2013
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Objectives: The aim of this study was to evaluate the Prevalence of Jaundice Based on Liver Function Test in Patients Attending OPD of Nepalgunj Medical College and Teaching Hospital. Materials and Methods: A hospital based study was carried out in Nepalgunj Medical College, Kohalpur, Banke Nepal to determine prevalence of jaundice. A total of 4280 subjects females and males were included in this study. The study took place from February 2012 to January 2013. Liver function tests were performed using serum levels of bilirubin, total protein,  albumin,  serum  glutamate oxaloacetate transaminase  (SGOT),  serum glutamate pyruvate  transaminase  (SGPT), and alkaline phosphatase (ALP) as indicators of liver function .The results obtained from the above investigation were analyzed and expressed as mean ± SD by using Excel 2007. The comparison was done by student t test on no. of variable of each parameter using SPSS version 16. Results: We investigated the prevalence of jaundice in 4280 subjects on the basis of liver function test. Out of 4280 subjects, 152 patients (3.55%) were selectively diagnosed as jaundiced and another 152 healthy individuals have taken as control. The serum concentrations of all the parameters of liver function test in jaundiced patients were significantly higher (p<0.0001) than those of controls except total protein and albumin which was significantly lower in (p<0.0001) experimental group than the control group. Conclusions: Therefore, from the trend of our data, we can conclude that liver dysfunction is a very common feature in the population of western part of Nepal this may be due most of the people are addicted to alcohol consumption. This need to be stopped and continuous surveillance for bilirubin test is very important to diagnose the jaundice.
RENAL FUNCTION TEST ON THE BASIS OF SERUM CREATININE AND UREA IN TYPE-2 DIABETICS AND NONDIABETICS Singh, P.; Khan, S.; Mittal, and R. K.
BALI MEDICAL JOURNAL Vol 3 No 1 (2014)
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Background: Type-2 diabetes mellitus has quickly become a global health problem due to rapidly increasing population growth, aging, urbanization and increasing prevalence of obesity and physical inactivity. Diabetic nephropathy is one of the major causes of chronic renal failure. Both serum urea and creatinine are widely used to assess the function of kidney. This study was conducted to observe the impaired renal function in type 2 diabetics and compare with non-diabetics controls. Method: To determine the incidence of renal dysfunction in diabetics in Nepalgunj medical college and Hospital , Nepalgunj , Banke, Nepal , blood samples from 100 diabetic subjects and 100 non-diabetic controls were taken between the period 1st February  , 2012  to  31st January , 2013 for investigation of  plasma glucose fasting(FPG), blood urea and serum creatinine. These biochemical parameters were determined by using a fully automated clinical chemistry analyzer. Results: Our findings showed that the level of blood urea (P<0.0001, 95%Cl) and serum creatinine (P?0.0004,95%Cl ) were significantly higher in type 2 diabetics as compared to non-diabetics in both male and female. There was no significant difference between diabetic male and female. 15 out of 100 diabetes samples have high urea level whereas 7 out of 100 had increased creatinine level. In control only 3 samples had high urea value and 1 had high creatinine level. There was statistical signi?cant increased in urea level with increased in blood sugar level. Conclusion: Blood urea and creatinine is widely accepted to assess the renal functions. Good control of blood glucose level is absolute requirement to prevent progressive renal impairment.
Co-Authors A.H. Siddiqui and R. K. Mittal R.K. Mittal S. Khan