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All Journal Jurnal Akuakultur Indonesia
Abdulgani Amri Siregar
Institut Pertanian Bogor, Indonesia Kampus IPB Darmaga Bogor 16680
Agriculture, Biological Sciences & Forestry

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Detection of Edwardsiella tardain catfish (Clarias sp.)by fluorescent antibody technique (FAT) Firma, .; Amalia, Rahman Rizky; Sari, Utami; Chusbul, Chotimah; Siregar, Abdulgani Amri
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1114.389 KB) | DOI: 10.19027/jai.11.96-102

Abstract

The fluorescent antibody technique (FAT) can be used to detectEdwardsiellatarda rapidly. As prelimenary step, it have been performed purity test of bacteria by PCR, specificity test of mouse anti-E. tarda monoclonal antibody by blocking using chicken and rabbit serum, and optimization of conjugate secondary antibody mouse IgG-H&L (FITC) dillution rate. Fourty eights catfish were intraperitoneally injected by 0,3 mL of E. tarda with different concentration, namely: 102 CFU/mL, 103 CFU/mL, 105 CFU/mL. Kidney, spleen, and liver from three fishes in each treatment were collected at interval time of six hours, 12 hours, 24 hours, 48 hours after infection. The results showed that E. tarda could be detected in fish infected with 102 CFU of E. tarda after six hours of injection in kidney,liver, and spleen of infected fish. Hence, FAT is faster than detection by bacterial culture method, and this technique can be useful to prevent the spread of fish disease. Keywords: fluorescent antibody technique, Edwardsiella tarda, detection, catfish
Co-Authors . Firma Chotimah Chusbul Rahman Rizky Amalia Utami Sari