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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Nurrahmi Dewi Fajarningsih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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AN EMERGING MARINE BIOTECHNOLOGY: MARINE DRUG DISCOVERY Nurrahmi Dewi Fajarningsih
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 2 (2012): August 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i2.19

Abstract

Marine natural resources offer an opportunity to discover a novel chemical diversity withinterest ing pharmacologically active compounds to treat many diseases such as cancer,inflammation, bacterial and parasitic infections, and many other diseases. Marine drug discoveryis a rising area in marine biotechnology. Several hits of marine-derived drug compounds wereapproved; two of them are Ziconotide and Trabectedin. In 2004, Ziconotide was approved as paintreatment drugs in the United States and Europe. Then, in 2007, Trabectedin was also approvedas anticancer drug in Europe. The main problem in marine drug discovery research is materialsupply problem. Up till now, strategies to overcome the problem are “Pharmaceutical aquaculture”of biologically active marine biota and chemical synthesis approach. Chemical synthesis approachis feasible solution to be used, especially when working with less complex structure of compounds.However, when working with structurally complex compounds where total or even semi synthesiswas very difficult to be provided, aquaculture can be a solution. Currently, the use of microbiology,biochemistry, genetic, bioinformatics, genomic and meta-genomic has been intensifying in orderto have a better result in marine natural product drug discovery. As chemical synthesis needs anexpensive investment of advanced technology and highly skilled human resources, thuspharmaceutical aquaculture is more practicable to overcome the material supply insufficiency inIndonesia. Up till now, many Indonesian marine bioprospectors have been working with culturablemarine microorganism to produce bioactive compounds and some others starting to work withgenomic and metagenomic-based drug discovery.
CYTOTOXIC ACTIVITY AND APOPTOSIS INDUCTION OF T47D CELL LINES BY Turbinaria decurrens EXTRACT Muhammad Nursid; nurrahmi dewi fajarningsih; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): May 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i1.78

Abstract

Marine algae is known to contain a wide variety of biomedical compounds having pharmaceutical applications. The aim of this research was to evaluate cytotoxic activity and apoptosis induction of Turbinaria decurrens extract on T47D cell lines.  Cytotoxic activity test was conducted by using MTT assay whereas detection of apoptosis was evaluated by DNA fragmentations and flow cytometry analysis. The MTT test showed that crude extract had medium cytotoxic activity to T47D, HepG2, and C28 cell lines with IC50 value of 172, againts 360 and 330 µg ml-1, respectively. After solvent partition of crude extract, the cytotoxic activity of n-hexane and ethyl acetate fractions T47D cell increased, the cytotoxic activity of n. hexane and ethyl acetate fractions T47D cell increased with  IC50  value of with IC50  43.1 and 51.9 µg ml-1, respectively, whereas IC50 value of methanol fraction was 383.0 µg ml-1. Analysis of DNA fragmentation of T47D cell showed that  both n-hexane and ethyl acetate fractions could not fragment DNA as a features of apoptosis. However, flow cytometry analysis by using annexin-V and propidium iodide staining revealed that n-hexane and ethyl acetate fractions could induce apoptosis in T47D cell. This research indicated that Turbinaria decurrens had potency to induce apoptosis in T47D cells.
Co-Authors Ekowati Chasanah Muhammad Nursid