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Irma Hermana
Research and Development Center for Marine and Fisheries Product Processing and Biotechnology
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Prevalence of Aflatoxin B1 in Commercial Dried Fish from Some Regions of Java Ninoek Indriati; Irma Hermana; Izhamil Hidayah; Endang Sri Rahayu
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.290

Abstract

The purpose of this study is to study the prevalence of aflatoxin B1 in commercial dried salted fish and other related information. A total of 150 samples were classified into 3 groups, based on the salt content. Low salt content (0-5%) were dried anchovies (Stolephorus sp.) and commerson’s anchovy (Stolephorus commersonii); moderate salt content (6-10%) were medan anchovy (Stolephorus bataviensis) and  whipfin silverbiddy (Gerres filamentosus) while high salt content (10%) were moonlight gouramy (Trichogaster microlepis) and snakehead fish (Channa striata). The samples were collected from different seller in Java Island and then determined for Aspergillus flavus, Aflatoxin B1, salt content, moisture content, pH, water activity and total mold count. Results showed that dried salted fish were contaminated with A. flavus at temperature of 25.2-32.2 oC, 65-84% humidity, 17-50% moisture content, 0.25-19.88% salt content, and 0.73-0.86 aw. The prevalence of A. flavus in dried salted fish was 9.33% (14/150) and the prevalence of aflatoxin B1 was 8% (12/150) with detectable concentrations of 10.71-33.6 ppb.
OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus lactis ssp. lactis CN1.10a ORIGIN FROM RUSIPS Ninoek Indriati; Arifah Kusmarwati; Irma Hermana
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 3 (2014): December 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i3.107

Abstract

Previous study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a  isolated from rusip, a traditional Bangkanese fermented fish product.The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2L). Followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity on that scale, and also stability of bacteriocin during storage.The results showed that the optimum temperature and pH for the growth of producer cell were 28°C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH2PO4, 0,2% NaCl and 0,02% MgSO4.7H20) in addition of sucrose as carbohydrate source. Based on the growth curve performedon CM medium with KH2PO4, the L. Lactis ssp lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was  8000 AU/mlat24th hour.Bacteriocin  was relatively stable when stored at -20°C for 1month with a relative activity of 69,4%.
Co-Authors Arifah Kusmarwati Endang Sri Rahayu Izhamil Hidayah Ninoek Indriati