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Mitra Slipranata
Department of Clinical Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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DETEKSI GEN PENYANDI ADHESIN PADA VEROCYTOTOXIGENIC Escherichia coli (VTEC) ISOLAT SAPI (Detection of Gene Encoding Adhesin of Verocytotoxigenic Escherichia coli (VTEC) Isolated from Cattles) Prihtiyantoro, Wahyu; Hartatik, Hartatik; Slipranata, Mitra; Sandi, Novra A.
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5135

Abstract

This study was aimed to perform phenotypic and genothypic characterization of Escherichia coli (E. coli), particularly VTEC strain isolated from cattle faeces. In this study, 25 E.coli isolated from faeces specimens and faeces base fertilizer of dairy and beef cattles were used. Examination were carried out using phenotypic and genothypic characterization which is specified for E coli VTEC strain. The result showed that 20 % samples of fresh faeces specimens were detected as VTEC strains and none of isolate was detected from faeces base fertilizer samples. From VTEC strains, could detect 16 % VT1 gene, 12 % VT2 genes and 8% of both. Detection on gene pyelonephritis-associated pilli (pap), S fimbrial adhesion (sfa), and afimbrial adhesion (afa) were found about 60%, 80% and 80%, respectively.
RESISTENSI ANTIBIOTIK DAN DETEKSI GEN PENGODE METHICILLIN RESISTANT Staphylococcus aureus (MRSA) ISOLAT BROILER DI WILAYAH YOGYAKARTA (Resistance of Antibiotics and Detection of Gene Encoding Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Broiler in Yogyakarta) K, Khusnan; Kusmanto, Dwi; Slipranata, Mitra
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3360

Abstract

The aims of the research were to evaluate resistance of Staphylococcus aureus against several antibiotics and to detect the gene encoding ofmethicillin resistant Staphylococcus aureus (MRSA) in broiler. Twenty three Staphylococcus aureus isolated from infected broiler in Yogyakartawere used. Genotypic identifications were done based on the occurrence of gene nuc and 23SrRNA. The resistance assay to several antibioticsrevealed that Staphylococcus aureus were resistant to penicillin 78% (18/23), doxycyclin 56% (15/23), gentamicin 26% (6/23), tetracyclin 22% (5/23), erythromycin 13% (3/23), and methicillin 9% (2/23). Detection of gene encoding MRSA on 23 isolats of S. aureus showed that 8 (34.8%) isolates contain gene mecA.Key words: Staphylococcus aureus, broiler, resistant, methicillin, mecA
IDENTIFIKASI DAN KARAKTERISASI FENOTIPE Staphylococcus aureus ASAL KASUS BUMBLEFOOT DAN ARTHRITIS PADA BROILER k, Khusnan; Prihtiyantoro, Wahyu; Slipranata, Mitra
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.332

Abstract

Penelitian ini bertujuan mengidentifikasi dan karakterisasi Staphylococcus aureus (S. aureus) yang berasal dari kasus bumblefoot dan arthritis. Dalam penelitian ini digunakan 10 isolat S. aureus yang terdiri atas lima hasil isolasi asal kasus bumblefoot dan lima hasil isolasi asal kasus arthritis pada ayam broiler. Identifikasi S. aureus dilakukan melalui uji fermentasi manitol salt agar (MSA), pewarnaan Gram, uji koagulase, clumping factor, dan uji katalase. Karakterisasi fenotipe S. aureus yang dilakukan meliputi sifat hemolisis pada plat agar darah, sifathidrofobisitas, dan kemampuan hemaglutinasi. Hasil penelitian menunjukkan bahwa semua isolat S. aureus mampu memfermentasi MSA, positif uji koagulase, clumping factor, dan katalase. Staphylococcus aureus mampu menghemolisis plat agar darah dengan memperlihatkan sifat -hemolisin (80%), -hemolisin (10%), and -hemolisin (10%). Semua isolat S. aureus (100%) bersifat hidrofil dan mampu mengaglutinasi sel darah merah kelinci.
Co-Authors Dwi Kusmanto, Dwi Fachry Abda El Rahman Khusnan k Sandi, Novra A. Wahyu Prihtiyantoro