Soeharjono Soeharjono
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia

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The Effectivity of Scirpus Grossus and Limnocharis Flava As Fitoremediation Agents of Nitrate-Phosphate To Prevent Microcystis Blooming In Fresh Water Ecosystem Aliyah Siti Sundari; Catur Retnaningdyah; Soeharjono Soeharjono
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

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The aims of this study were to reduce the concentration of dissolved nitrate-phosphate, furthermore it can inhibit the growth and prevent the occurrence of Microcystis bloom waters. The study was conducted experimentally in the laboratory. The Microcystis isolation carried out in Sutami reservoir. Then, remediation treatment with hydromacrophyte (Scirpus grossus, Limnocharis flava and combination of both hydromicrophyte) were done during 15 day incubation period. Abiotic factors were measured on day 0, 6, 12 and 15, but the abundance of Microcystis cells was counted daily. The productivity of hydromacrophyte was measured at the end of the research. The research results showed that hydromacrophytes were effective to reduce nitrate and phosphate concentrations. Combination of S. grossus and Microcystis reduced nitrate concentration up to 99.89 %, while highest reduction of dissolved phosphate (98.22 %) carried out by combination of L. flava and Microcystis. The combination treatment of L. flava and S. grossus was capable to prevent Microcystis growth rate and carrying capacity that is 65 cells.L-1.day-1 and 6.93 x 104 cells/mL.
The Capability of Equisetum ramosissium and Typha angustifolia as Phytoremediation Agents to Reduce Nitrate-Phosphate Pollutants and Prevent Microcystis Blooming in Fresh Water Ecosystem Viky Vidayanti; Catur Retnaningdyah; Soeharjono Soeharjono
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

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The aim of this study is to find out the kind of hydromacrophyte composition having the highest capability to reduce nitrate-phosphate pollutant and Microcystis growth in phytoremediation media using Equisetum ramosissium, Typha angustifolia and the combination of both. Microcystis were obtained from Sutami reservoir, then being inoculated in a media grown by hydromacrophytes (E. ramosissium, T. angustifolia and both of the hydromacrophytes) in the batch culture system. The number of Microcystis was counted every day within fifteen days. Abiotic factors were observe, including the concentration of nitrate using brucine-colorimetry method and orthophosphate (dissolved phosphate) using stannous chloride-colorimetry method on day 0, 6th, 12th and 15th. The growth rate of Microcystis carrying capacity and orthophosphate-nitrate levels among treatments were analysed by ANOVA test. The results showed that E. ramosissium and T. angustifolia in mono and polyculture techniques had similar potentiality to reduce the nitrate and ortophosphate. The concentrations of nitrate and orthophosphate decreased over 70 % in the 6th day after incubation. All of the treatments were able to reduce the carrying capacity of Microcystis up to 46 % , but the growth rates were similar in all media, that is, around 97-170 cells/L/day. Keywords: Equisetum ramosissium, Microcystis, nitrate, phosphate, Typha angustifolia