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All Journal Borneo Journal Of Pharmascientech
Dewi Puspa Lotulung
Universitas Pancasila Jakarta
Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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ISOLASI DAN BIOINHIBITOR AKTIVITAS ENZIM TIROSINASE DARI EKSTRAK ETIL ASETAT PADI HITAM (Oryza Sativa L Indica) Agus Rochmat; LBS Kardono; Dewi Puspa Lotulung
BORNEO JOURNAL OF PHARMASCIENTECH Vol 3 No 2 (2019): Borneo Journal of Pharmascientech
Publisher : Sekolah Tinggi Ilmu Kesehatan Borneo Lestari Banjarbaru

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Abstract

ABSTRACT Melanin is a color pigment in the skin, hair, eye choroid layers, and certain tumor cells. The process of melanin formation in the human body can reduced by several mechanisms, such as antioxidants, tyrosine enzyme inhibitors, and hormonal activity. The process of formation of melanin or pigment in human skin occurs with the help of biocatalysts (enzymes) and ultraviolet (UV) rays found in the sun. Biocatalysts that play a role in this browning reaction are tyrosine which can found in animals, plants and humans. The process of extracting black rice using methanol then continued by separation using column chromatography. The yield of ethyl acetate fraction was 3.26%. The highest yield was 4.1 mg with the characteristics: maximum wavelength of UV light at 298 nm, the name of the isovanillic acid compound and IC50 tyrosine inhibition test of 107.91 ppm Keywords: black rice, tyrosine enzym, isovanillic acid
Co-Authors Agus Rochmat LBS Kardono