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All Journal BERITA BIOLOGI Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences)
Djoko Soetrisno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Veterinary

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INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Sapsuha, Yusri; Soetrisno, Djoko; Kustantinah, Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1729.405 KB) | DOI: 10.14203/bb.v10i5.1921

Abstract

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33±11.50.
PRODUKSI BAHAN KERING DAN TINGGI TANAMAN RUMPUT SUDAN (Sorghum sudanense) PADA DEFOLIASI KE DUA TANPA PUPUK NITROGEN ULANGAN Soetrisno, Djoko; Yoku, Onesimus
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 5 No 1 (2010): JURNAL ILMU PETERNAKAN
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.952 KB)

Abstract

The aim of this study were to obtain the dry matter (DM) production of sudan grass at second harvest under different plant distances without nitrogen fertilization and to decide whether fertilization have to be added or not. The design of the study was split plot design 4 x 7 with three blocks. The main plots were plant distances, namely :20x40 cm (J1), 40x40 cm(J2), 60x40 cm (J3) and 80x40 cm (J4), and each plot was divided into 7 subplots for randomly N application treaments, namely :N0 (P0); N75 (P1); N150 (P2); N225 (P3); N300 (P4); N375 (P5) dan N450 (P6). Phosphorus and K fertilizer were given at the same rate, that were 300 kg P2O5 and 150 kg K2O. Result of the study showed that planting distances, level of fertilizers and the interaction were significant (P<0.05) affected DM production and height of plants in the second harvest. The DM production in the second harvest was significantly lower (P<0.05) than the DM production in the first harvest. From this study it can be concluded that N fertilizer application should be given again after cutting in order to maintain the DM production of sudan grass in the next harvest.
PRODUKSI RUMPUT SUDAN DAN PERHITUNGAN EKONOMI PENGGUNAAN PUPUK NITROGEN, FOSFOR, DAN KALIUM Yoku, Onesimus; Soetrisno, Djoko; Siradz, Syamsul Arifin
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 5 No 2 (2010): JURNAL ILMU PETERNAKAN
Publisher : Fakultas Peternakan Universitas Papua

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Abstract

The aim of this study were to obtain the optimum level of NPK fertilizers of sudan grass and economic of NPK fertilizers utilization. The randomized completely block design (RCBD) were used to evaluate, namely 4 levels of fertilizer doses NPK and 4 blocks for determine the grass production. The treatments for randomly N application, namely: No (PO); N150 (P1); N300 (P2); and N450(P3). Phosphorus and K fertilizer were given at the same rate, that were 300 kg P2O5 and 150 kg K2O ha-1. The results of study showed that DM production for PO (3.19 tonha-1) differed significantly with P1, P2 and P3; but between P2 and P3 did not differ significantly. There were increased DM production due to increasing of fertilizers applied. The values of DM production increasing percentage for P1, P2, and P3 compared with PO, for DM were 69.44%, 80.45% and 85.28%, respectively. The increased percentages of DM production of P3 tended to decrease. Therefore, it can be summarized that P2 (300 kg N, 300 kg P and 150 kg K ha-1) was optimum level for DM production. Based on partial budget analysis, indicated that cultivation of sudan grass with NPK fertilizer treatments yielded profit positively. The profit value for P1, P2, and treatment of P3 were Rp 581.500,-; Rp 2.009.500,-; and Rp 2.997.500,-per hectare, respectively.
Co-Authors Kustantinah Kustantinah Onesimus Yoku Ristianto Utomo Siradz, Syamsul Arifin Yusri Sapsuha