IDA HANARIDA SOMANTRI
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Jln. Tentara Pelajar No. 3 A Bogor

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Kultur Antera, Teknik Penyelamatan Embrio dan Rekayasa Genetik untuk Menunjang Pemuliaan Tanaman Padi Somantri, Ida Hanarida; Ambarwati, A. Dinar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 3 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1205.575 KB) | DOI: 10.24831/jai.v29i3.1544

Abstract

The success of biotechnology in developed country encourage Indonesia to use it in rice breeding program. Every technick In biotechnology has advantage and it can be used to solve a problem in a certain purpose. Embryo rescue technich solved the problem of crossing with wild species. This technick may insert a gene from one organism to another through gene  transformation technich. The anther culture technich been used on rice: Javonica, Javanica and indica. Generally, the percentage of callus induction and regeneration has great variation (1.8% - 40%). It was Influenced by the number and type of genome. The transformation of rice using microprojecty system using cry gene resulted 172 putative transgenic plants. Twenty five of them have been tested using moleculer technick and it showed t at the plants contained cry gea.   Key words: Anther culture, Embryo rescue, Rice