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I Putu Suastika
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STUDI HISTOLOGI LIMPA SAPI BALI Ni Luh Eka Setiasih; Ni Ketut Suwiti; I Putu Suastika; I Wayan Piraksa; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The objective of this study to find out the histological of spleen of bali cattle. Thespleenwere collected from 20 bali cattle in Pesanggaran abattoir, Denpasar and thenevaluated microscopically. Histological findings were assessed by H-E stainpreparations.Microscopically, showed that the spleen of bali cattle consists of capsule,white and red pulp. The capsule composed of connective tissue and smooth muscle withthick of these layer is 24,3 ± 3,7 ?m. The white pulp subdivided into the marginal zone andthe follicle. It is composed of reticulocyte (lymphocytes and macrophages) andreticular fibers. The red pulp composed of arterioles, capillary, and vascular sinusoidswhich are large numbers of erythrocytes, macrophages, dendritic cells, plasma cells andsparse lymphocytes.
Pengaruh Pemberian Pegagan (Centella asiatica) terhadap Gambaran Mikroskopis Limpa Mencit yang Diinfeksi Salmonella typhi I Gede Oka Budiawan; Ni Ketut Suwiti; I Putu Suastika; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak pegagan terhadap gambaran histologis limpa mencit yang diinfeksi Salmonella typhi. Mencit sebanyak 24 ekor dibagi empat kelompok, yakni kelompok I (P0) sebagai kontrol diberikan aquades steril, kelompok II (P1) diberikan pegagan dengan dosis 125 mg/kg bb, kelompok III (P2) diberikan pegagan 250 mg/kg bb, dan kelompok IV (P3) diberikan pegagan 500 mg/kg bb. Setiap perlakuan diulang sebanyak 6 kali. Pegagan di berikan setiap hari selama 14 hari. Setelah 14 hari seluruh mencit diinfeksi S.typhi. Pada hari ke-15 dilakukan nekropsi untuk pengambilan sampel berupa limpa dan dibuat preparat histologi. Pengamatan preparat dilakukan di Laboratorium Histologi, meliputi persentase nekrosis. Metote pewarnaan menggunakan Haematoxylin Eosin (HE). Data yang diperoleh dianalisis dengan Uji Kruskal-Wallis. Hasil analisis menunjukan gambaran  mikroskopis limpa mencit yang diberikan pegagan dosis 500 mg/kg bb berbeda nyata (P<0,05) dengan limpa mencit yang diberikan pegagan dosis 125 mg/kg bb, 250 mg/kg bb dan mencit yang tidak diberikan pegagan.  
STUDI HISTOLOGI USUS BESAR SAPI BALI Ni Ketut Suwiti; Ni Luh Eka Setiasih; I Putu Suastika; I Wayan Piraksa; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

A Study to detect the microscopic structure of large intestine (caecum, colon, rectum) ofbali cattle has been carried out. The samples were collected from 20 of bali cattle in Pesanggaran abattoir, Denpasar. The tissue samples were fixed, dehydrated and embeddedin paraffin and 4-5 mm sections. Harris-Haematoxilin-Eosin staining method, using toidentified of histological structure. Microscopic analysis was performed using binocularlight microscope (100 x, 450x). The study showed that the wall of the caecum, colon, andrectum are made up of four layers : mucosae, submucosae, muscularis externa, and serosa.The lined by mucosae ephitelium of simple columnar cells. The thick of mucosaare 29,9 ± 5,0 mm (caecum) 37,5 ± 8,4 mm (colon) and 58,9 ± 9,5 mm (rectum)respectively. The submucosae contain connective tissue and thick of these layerare 54,8 ± 8,8 mm; 21,2 ± 7,1 mm; and 16,5 ± 4,5 mm respectively. The muscularis externaconsists of two layers of smooth muscle inner circular and outer longitudinal. The thick ofthe mucularis externa are 98,5 ± 15,4 mm; 166,9 ± 44,4 mm; and 479 ± 28,6 mmrespectively. The serosa forms the outermost layer with thick 29,5 ± 8,5 mm;20,9 ± 7,6mm; and 12,1 ± 3,6 mm respectively. We observed for the presence richof Goblet cells and few limphatic nodules (Peyer’s patches) but no showed villi.
Studi Histologi dan Histomorfometri Daging Sapi Bali dan Wagyu (HISTOLOGYCAL AND HISTOMORPHOMETRY STUDY OF BALI CATTLE AND WAGYU BEEF) Ni Ketut Suwiti; I Putu Suastika; Ida Bagus Ngurah Swacita; I Nengah Kerta Besung
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to detect histological structure and histomorphometry of bali cattleand wagyu beef. The samples were collected from rib eye muscle of wagyu and bali cattle. The tissuesamples were fixed, dehydrated and embedded in paraffin and 4-5 mm sections, for histological examinationand stained with Haematoxilin-Eosin (HE). The light binocular microscope was used to identify the samples.The study showed that the beef histological structure consisted of: cells / muscle fibers, fatty and connectivetissues. The muscle fibers of wagyu were observed larger, regular, and longer compared to the Bali cattle.Additionally, intramuscular fat cells and connective tissue interlobulus were found less and thinner thanthe Bali cattle. In the area of muscle where the longitudinal cut was done, its observed that the presence ofI and A bands in Bali cattle beef were clearer compared to the wagyu. The diameter muscle fibers of balicattle beef (45.00 ±1.94 ?m), was smaller (P < 0.01) compared to the wagyu (75.00±1.82 ?m), as well as fatcell size of bali cattle beef (90.10 ±2.13 ?m) and wagyu beef (195.00±2.57?m). In conclusion, the structureof histology and histomorphometry of rib eye muscle in Bali cattle were different to the wagyu.
Co-Authors I Gede Oka Budiawan I Wayan Piraksa Ida Bagus Ngurah Swacita Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Nyoman Werdi Susari