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All Journal INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner
Ketut Suata
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Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

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Bioaktivitas Forbazol-E terhadap Kerusakan Ultrastruktur Dinding Sel Staphylococcus aureus Ni Putu Ristiati; Ketut Suata; Dewa Ngurah Suprapta
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The bioactivity role of phorbazol-E in inhibiting bacterial protein synthesis is unknown. Onthe contrary, phorbazol-A, B, C, and D have been proved to inhibit the bacterial protein synthesis.The effect of forbazol-E on Staphylococcus aureus ATCC25922 cell wall ultrasctucture destructionwas observed. The study used randomized-post test-only control group design which consist of threetreatment (control, treatment I and II) with 9 repeatation. Treatment I: 1 ml overnight culture ofS. aureus was cultured into a mixed of 20 ml Mueller Hinton (MH) broth and 37.5 mg/L phorbazol-E; whereas in treatment II phorbazol-E used was 75.0mg/L. The growth curve of S. aureus wasmonitored using spectrophotometer; whilst the destruction of cell wall ultrastructure was observedusing Transmission Electron Microscope (TEM) at Eijkman Insitute, Jakarta. Phorbazol-E at75.0mg/L significantly caused the destruction of the bacteria cell wall ultrastructure and inhibitedthe bacteria growth in comparison to phorbazol-E at 37.5 mg/L (p<0.05).
Respon Antibodi Antikapsid pada Mencit yang Divaksin Vaksin Limpa dan Vaksin Kultur Virus Penyakit Jembrana Ni Luh Putu Manik Widiyanti; Ketut Suata; Nyoman Mantik Astawa; Hartaningsih -
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle are one of Indonesia national asset which need to be conserved as they have many advantages.They are however susceptible to many infections diseases such as jembrana disease. Currently, thedisease is prevented by vaccination using vaccine derived from jembrana disease virus (JDV)-infected balicattle. An alternative vaccine using JDV-infected lymphocyte culture is expected to increase the virusyield and is therefore likely to increase the antibody response in the vaccinated animals. A study thereforewas therefore conducted to compare the anti-capsid antibody response of Balb/c mice immunized withvaccine derived from the spleen of infected cattle (spleen vaccine) and those immunized with vaccinederived from infected lymphocyte culture (culture vaccine). As many as 16 female Balb/c mice weredivided into two groups, Each group was vaccinated 4 times weekly respectively with spleen and culturevaccines. The antibody response against the capsid protein of JDV was determined using enzyme-linkedimmunosorbent assay (ELISA) and the absorbance reading of mice sera from each group was compared. Tstudentand univariate analysis showed that the average absorbance reading sera of sera derived frommice vaccinated with spleen vaccine (0.15) was not significantly different from those vaccinated withculture vaccine (0.18). It appears that culture vaccine is able to induce anti-capsid antibody response ashigh as spleen vaccine.
Co-Authors AAG Sudewa D. Dewa Ngurah Suprapta HARTANINGSIH - I Ketut Suastika I Made Bakta I NYOMAN MANTIK ASTAWA Ketut Suega Made Wardhana NI LUH PUTU MANIK WIDIYANTI Ni Putu Ristiati