Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Jurnal Veteriner
HARTANINGSIH -
Unknown Affiliation
Medicine & Pharmacology Veterinary

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
CENTELLA ASIATICA EXTRACT INCREASED ON THE LEVEL OF INTERLEUKIN 6 (IL-6) IN MICE Kerta Besung, I Nengah; Mantik Astawa, Nyoman; Suatha, I Ketut; -, Hartaningsih
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 5, No. 1 Januari 2011
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.36 KB)

Abstract

Salmonellosis is still problem in many developing countries including Indonesia. Themain problem in controlling and handling the disease is that only few antibiotics are availableto cure the disease. In addition, the prolonged use of such antibiotics often lead to bacterialresistant against the antibiotics. A herbal drugs such as Centella asiatica (in Indonesia isknown as pegagan) contains triterphenoid saphonins which acts as immunostimulant capableof enhancing the phagocytic activity of macrophages. However, no study has been conductedto investigate the use pegagan in activating macrophage of mice infected with Salmonellatyphi. A study was therefore conducted to find out the ability of Cantella asiatica inenhancing on the level interluekine (IL)-6 following challenge with Salmonella typhi. It istherefore expected that herbal drug such as Cantella asiatica can be used as an alternativemedicine to prevent and cure salmonellosis in both animals and human.Experimental laboratory studies were conducted using Completely FactorialRandomized Design. Mice were divided into 4 groups and they were treated respectively withdestilated water (negative control), 125, 250, and 500 mg/kg bw of Centella asiatica extract.The treatment was conducted daily for 2 weeks and the mice were then inoculated with 105cells of S. typhi. The level of IL-6 response were examined 24 hours, 2 weeks, and 4 weeksafter inoculation with S. typhi.The result showed that treatment of mice with Centella asiatica extract significantly(p<0,05) enhaced IL-6 level of Balb/c mice following inoculation with S. typhi. The highestIL-6 level were observed in mice treated Centella asiatica extract at the dose of 500 mg/kgBW (385,9257±125,4314 pg/ml serum). And the highest IL-6 level were observed at 2 weeksfollowing inoculation with S. typhi (533.4262 ± 81.7184 pg / ml).A further study is recomended to examine the celluler immune response and moredetailed study on the humoral immune response of animals or human before this herbal isused as alternatif medicine to prevent and cure typhoid fever. It is also important to study thebest preparation, the half life, and the side effect of Centella asiatica in human and animals.
RESPON KEKEBALAN HUMORAL MENCIT BALB/C YANG DIVAKSINASI DENGAN VAKSIN LIMPA DAN VAKSIN KULTUR PENYAKIT JEMBRANA TERHADAP PROTEIN VIRUS JEMBRANA MANIK WIDIYANTI, NI LUH PUTU; SUATA, I KETUT; MANTIK ASTAWA, I NYOMAN; -, HARTANINGSIH
INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES Vol. 2, No. 2 Mei 2008
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.306 KB)

Abstract

Sapi bali adalah salah satu aset nasional Indonesia yang harus dilestarikan karena mempunyai keuntungan ekonomi. Tetapi sapi bali mempunyai beberapa kelemahan penyakit khususnya penyakit Jembrana yang disebabkan oleh virus penyakit Jembrana (JDV). Pencegahan terhadap penyakit Jembrana telah dilakukan dengan vaksinasi. Vaksin yang terbukti dapat menurunkan tingkat kematian sapi bali terserang JDV adalah vaksin limpa. Jenis vaksin ini hanya mampu menginduksi kekebalan dengan perlindungan 70%. Proteksi ini dapat ditingkatkan jika jumlah virus yang digunakan dalam vaksin meningkat. Tekhnik kultur in vitro adalah salah satu metode meningkatkan jumlah virus penyakit Jembrana, dan selanjutnya dibuat vaksin kultur. Hasil penelitian menunjukkan bahwa sel limfosit sapi bali terinfeksi JDV adalah 9,5% pada limpa dan 57,43% pada sel kultur. Uji westernimmunoblotting sel limfosit sapi bali dari darah tepi dan limpa terinfeksi JDV menggunakan antibodi monoklonal (AbMo) anti Ca, terdeteksi protein dengan berat molekul 26 kDa, 42 kDa dan 51 kDa. Pada medium kultur PBMC dan endapan plasma sapi bali terinfeksi JDV, teridentifikasi protein dengan berat molekul 16 kDa an 26 kDa menggunakan AbMo, dan teridentifikasi protein dengan berat molekul 16 kDa; 21,5 kDa. 26 kDa; 29,7 kDa; 40 kDa dan 50 kDa menggunakan AbPo. Uji Elisa didapatkan nilai absorban antibodi mencit balb/c yang divaksinasi dengan vaksin kultur penyakit Jembrana lebih tinggi yaitu sebesar 0,3089 dibandingkan vaksin limpa yaitu sebesar 0,177 dengan p<0,05. Nilai absorban antibodi mencit balb/c terhadap antigen Ca, SU dan tat, memperlihatkan nilai absorban terhadap antigen SU berbeda sangat signifikan dibandingkan dengan antigen Ca dan antigen tat (p<0,01). Antigen Ca berbeda signifikan terhadap antigen tat (p<0,05).
Respon Antibodi Antikapsid pada Mencit yang Divaksin Vaksin Limpa dan Vaksin Kultur Virus Penyakit Jembrana Ni Luh Putu Manik Widiyanti; Ketut Suata; Nyoman Mantik Astawa; Hartaningsih -
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.052 KB)

Abstract

Bali cattle are one of Indonesia national asset which need to be conserved as they have many advantages.They are however susceptible to many infections diseases such as jembrana disease. Currently, thedisease is prevented by vaccination using vaccine derived from jembrana disease virus (JDV)-infected balicattle. An alternative vaccine using JDV-infected lymphocyte culture is expected to increase the virusyield and is therefore likely to increase the antibody response in the vaccinated animals. A study thereforewas therefore conducted to compare the anti-capsid antibody response of Balb/c mice immunized withvaccine derived from the spleen of infected cattle (spleen vaccine) and those immunized with vaccinederived from infected lymphocyte culture (culture vaccine). As many as 16 female Balb/c mice weredivided into two groups, Each group was vaccinated 4 times weekly respectively with spleen and culturevaccines. The antibody response against the capsid protein of JDV was determined using enzyme-linkedimmunosorbent assay (ELISA) and the absorbance reading of mice sera from each group was compared. Tstudentand univariate analysis showed that the average absorbance reading sera of sera derived frommice vaccinated with spleen vaccine (0.15) was not significantly different from those vaccinated withculture vaccine (0.18). It appears that culture vaccine is able to induce anti-capsid antibody response ashigh as spleen vaccine.
Co-Authors I KETUT SUATA I Ketut Suatha I NYOMAN MANTIK ASTAWA Ketut Suata NI LUH PUTU MANIK WIDIYANTI