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Pradipto Subiyantoro
RSUD Dr. Moewardi
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Perbedaan pH Saliva antara Remaja Wanita Pre Menarche dengan Remaja Wanita Post Menarche Syamsudduha, .; AR, Risya Cilmiati; Subiyantoro, Pradipto
Nexus Kedokteran Klinik Vol 2, No 2 (2013): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Riskesdas (riset kesehatan dasar) in 2007 result that women get more curative treatment of tooth healt. One factor that influence tooth healt is pH saliva. This research aims to know the difference of salivary pH between young women of pre menarche with young women of post menarche.. Methods: This research was an observational research using cross sectional approach and had been done in UNS Medical Faculty, TPA al-falah, and TPA al-ikhlas. Data was collected by using purposive random sampling method devide into 2 group (1.) pre menarche group, (2.) post menarche group. The subjek of first group is student of medical faculty of 2008, 2009, and midwifery 2011 UNS and second group is pupil of TPA al-Ikhlas and TPA al-Falah recidency of Surakarta. Two of group which a fullfil of inklusion and exclusion criteria compare of the pH. The data analysis used compare means independent-samples T-test  SPSS 20.0 for Windows. Results: this research shows (1) average salivary pH in pre menarche group is 6,867 dan post menarche group is 7,240. (2) results of compare means independent-samples T-test shows p = 0,00. Conclusions: This study found the difference of salivary pH between young women of pre menarche with young women of post menarche. Keywords: : salivary pH of pre menarche, salivary pH of post menarche, status of menstruation.
Deteksi Gen P14ARF Pada Spesimen Blok Parafin Lidah Penderita Karsinoma Sel Skuamosa Di RSUD Dr. Moewardi Rakhmat, Jinan Fairuz Anindika; Subiyantoro, Pradipto; Nirmala A, Vita
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background : The oral cavity cancer is twenty most common cancer that occurs in the world. Around  90%  oral cavity cancer cell carcinoma is squamous and most of the incidents come from tongue. p14ARF gene is one of the tumor suppressor gene that can prevent the occurrence of a tumor. The purpose of this research was to know the existence of p14ARF gene in paraffin block specimens of the tongue of the squamous cell carcinoma patients in the Regional Public Hospital Dr. Moewardi. Methods : This study was explorative and descriptive research. Paraffin block samples of the tongue squamous cell carcinoma patients were used in this study. The object of this research was p14ARF gene located on chromosomes 9p21. The samples were separated from the Paraffin Block and their DNA were isolated. The DNA products, then, were amplified by PCR and their PCR products were run in agarose gel electrophoresis. Later, the gel was visualized with Gel Documentation and analyzed to detect the  p14ARF gene. Results : On the visualisation of the gel product, the p14ARF gene was detected in one sample with atypical band pattern and it was not detected on the eight other samples. Conclusion : Only one sample of the tongue squamous cell carcinoma patients which was positive expressed p14ARF gene with atypical band pattern while the eight other samples were not. Keywords : tongue squamous cell carcinoma, p14ARF, oral cavity cancer 
Deteksi Gen P16 pada Sampel Blok Parafin Lidah Penderita Karsinoma Sel Skuamosa Rongga Mulut di RSUD Dr. Moewardi. Ernestine, Coraega Gena; Subiyantoro, Pradipto; Nirmala, Vita
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Oral squamous cell carcinoma is a part of the cancer in the head and neck which ranks 10th most cancers in the world with a wide geographical distribution. It is usually found in the late stage. P16 gene (CDKN2a/INK4a) is an important tumor suppressor gene  in carcinogenesis. This study aimed to detect the expression of p16 gene on paraffin block samples of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. Methods: DNA product used in this study was isolated from 14 samples on Paraffin Blocks of the tongue of the oral squamous cell carcinoma patients in RSUD Dr. Moewardi. The DNA products, then, were used as a template for PCR amplification of the p16 gene. Primers were designed with the base sequences deposited in Gene Bank. PCR amplification products were run in agarose gel electrophoresis and visualized to detect the presence of the p16 gene. Later, the results were analyzed and presented in descriptive data. Results: Only 6 of 14 samples were diagnosed as oral squamous cell carcinoma. From 6 samples only one was detected positive expression of p16 gene. DNA bands were detected in 3% agarose gel. The intensity of DNA bands was visualized lowly and there was a smear. On the 5 other samples, DNA bands were detected under the marker, thus they showed a negative result or the expression of p16 gene was not detectable. Conclusions: Among 6 samples of the tongue of the oral squamous cell carcinoma patients, only one showed positive expression of p16 gene. Errors in the molecular and tumor differentiation level analysis was able to cause the negative results in this study. Therefore further research that detected expression of p16 gene on the tongue of the oral squamous cell carcinoma patients was needed. Keywords:P16 gene, Paraffin Block, oral squamous cell carcinoma.
Deteksi Gen E-Cadherin Pada Karsinoma Sel Skuamosa Rongga Mulut dari Sampel Blok Paraffin ., Nabiel; Subiyantoro, Pradipto; A, Vita Nirmala
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background : Oral cavity cancer is the most cancer type often found across the globe. The treatment and the progonosis of this cancer have a very good chance to succeed but otherwise the spread of the cancer cell (metastasis) has a very high extent on them. There are numerous cases to be found in metastasis process. This research aimed to detect E-Cadherin gene in oral cavity squamous cell carcinoma from paraffin block samples. Methods : This research used a DNA product which resulted from DNA isolation of 14 parafin block samples. First, the products was amplified using PCR. Second, the PCR products were run in a gel electrophoresis to find out E-Cadherin gene on each sample. Results : Twelve samples that were diagnosed as squamous cell carcinoma and amplified by PCR as well as run in gel electrophoresis using 2% of agarose showed positive bands of E-Cadherin gene. Otherwise, the same samples run in gel electrophoresis using 3% of agarose showed no positive band of E-Cadherin gene. Summary : Twelve samples that were diagnosed as squamous cell carcinoma of oral cavity showed positive bands of E-Cadherin gene in 2% of agarose and no one positive band of E-Cadherin gene in 3% of agarose. Keywords : E-Cadherin gene, paraffin blocks, squamous cell carcinoma of oral cavity 
Co-Authors . Syamsudduha Coraega Gena Ernestine Jinan Fairuz Anindika Rakhmat Nabiel . Risya Cilmiati AR Vita Nirmala Vita Nirmala A Vita Nirmala A