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YB Subowo
Bidang Mikrobiologi, Puslit Biologi – LIPI

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STUDI BUDIDAYA JAMUR Lactarius sp. DI WAMENA, IRIAN JAYA Latupapua, HID; Subowo, YB
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.192 KB) | DOI: 10.14203/beritabiologi.v4i4.1272

Abstract

Research on the cultivated of mushroom of Lactarius gp.had been conducted in Wamena,Jayawijaya district of Irian Jay a. There were 6 kinds of media which consisted of 2 kinds of sawdust wood (Nothofaeus sp.and Paraserianthes falcataria).Each sawdust wood added in 3 level of nutrition (0%, 50% and 100%).The experimental design which had been used was factorial which arranged with completely randomized design by using 10 replications.The result showed that the micellia had grown on all of media.However,the fruiting body formation occured on the media which made of P.falcataria sawdust added in 0%; 50% and 100% nutrition and on the Nothofagus sp sawdust added in 100% nutrition.The highest yield of fruiting body had been showed on P. falcataria sawdust medium added in 100% nutrition.
PENGARUH BOBOT DAN KOMPOSISI MEDIA, RANGSANGAN SUHU DAN KIMIAWI TERHADAP PEMBENTUKAN TUBUH BUAH JAMUR SHIITAKE {LenHnus edodes [The Effect of Weight and Composition of Media, Temperatures and Chemically Stimulation on Fruiting Body Formation of Shiitake] Subowo, YB; Latupapua, HJD
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1268

Abstract

Research on the effect of weight and composition of media, temperature and chemically stimulation on fruiting body formation of shiitake (Lentinus edodes) had been done in Wamena, Jayawijaya district, Irian Jay a. The research consisted of three experiments. The first experiment consisted of 3 kinds of media such as media A, B and C(A- sawdust and 100% standard nutrition; B = sawdust and 50% standard nutrition; and C = sawdust without nutrition) and three kinds of temperatures were 20°C (room temperature),4°C (refrigerator temperature) and -10°C(freezer temperature).The second experiment used 4 kinds of media. They were media D, E, F and G which were stimulated by SnCh, CoCh, salicilic acid and the extract of bark of "Seno" (Castanopsis acuminatissima).The third experiment used medium B, weight 500 g and 1000 g (medium H and I).The result showed that medium H& I produced the highest fruiting body. However medium I produced the higher fruiting body compare to medium H).The added of the extract of the bark of C. acuminatissima.SnCh, CoCh and salicilic acid stimulated the fruiting body formation.
SELEKSI JAMUR TANAH PENGURAI LIGNIN DAN PAH DARI BEBERAPA LINGKUNGAN DI BALI [Selection of Lignin and PAHs Degrading Fungi from Some Environment in Bali] Subowo, YB; Corazon, Corazon
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1976

Abstract

An investigation on the selection of soil fungi that degrade lignin and PAHs from some environment in Bali had been done. Some soil fungi are able to degrade lignin compound and PAHs. This fungus can be used to degrade industrial waste containing lignin and hydrocarbon compound. The aim is to obtain fungal capable of degrading lignin and hydrocarbon compounds.The soil samples were taken from mangrove areas, beaches and organic farms to the laboratory for fungal isolation.The isolation of the sample was obtained 20 number isolates. Penicillium sp KSt3 is the highest yield of mycelium.This fungus has ability on poly R-478 degrading as much as 2.3% within 60 minutes. This fungus has laccase activity of 3,32 units/ml and capable of degrading phenanthren (PAH) as much as 85.8% in 8 days.
ISOLASI DAN SELEKSIJAMUR APHYLLOPHORALES PENGURAI LIGNIN DIHUTAN BUKIT BANGKIRAI, KALIMANTAN TIMUR Subowo, YB
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.277 KB) | DOI: 10.14203/beritabiologi.v9i6.857

Abstract

Isolation and selection of Aphyllophorales as lignin degrading fiingus had been done in Bukit Bangkirai Forest, East Kalimantan. Aim of the research was to obtain Aphyllophorales fungi which have ability to degrade lignin. The fresh fruit bodies were collected from trunks, branches and litters of plants in the forest. The work was conducted at dry season. Fruit bodies were taken to base camp for isolation. The isolates were tested for their ability to degrade lignin. We found 24 isolates of Aphyllophorales fungi. Those isolates were grew in poly R-478 medium, 12 isolates were able to degrade lignin. Megasproporia sp. strain IB-24 had the highest ability in lignin degradation. The fungus can degrade poly R-478 8,05% during 90 minutes.
UJI AKTIFITAS ENZIM SELULASE DAN LIGNINASE DARI BEBERAPA JAMUR DAN POTENSINYA SEBAGAI PENDUKUNG PERTUMBUHAN TANAMAN TERONG (Solarium melongena) Subowo, YB
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (904.655 KB) | DOI: 10.14203/beritabiologi.v10i1.2044

Abstract

The objective of this study was to obtain effective and beneficial microorganism specially fungi, which are able to stimulate and increase eggplant growth. Four lignocellulolytic fungi {Mucor sp. M13T, Aspergillus sp. M2P, Penicillium sp.M3P1, Penicillium sp. R 7.S and mixture of them) were evaluated for their ability to promote plant growth through provision of availability of soluble carbon finally stimulate beneficial microbial growth, and thus promoting eggplant growth. Aspergillus sp. M2P having cellulolytic activity (0.0612 U/ml) and Penicillium sp. M3P1 having ligninase capacity showing different plant growth stimulation on character and yield coefficient. Penicillium sp. M3P1 more effective to increase plant growth than that of control.While Aspergillus sp. M2P was less effective compared to Penicillium sp. M3P1. Therefore,we recommended using Penicillium sp. M3P1 as plant growth promoter for eggplant.