Mellisa Sim
Universitas Prima Indonesia

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Efek Penambahan Nanokitosan 1% Kedalam Berbagai Konsentrasi Ekstrak Kulit Kelengkeng Streptococcus Mutans Titania Aurely Tandra; Sabrina Khairunissa; Mellisa Sim; Florenly Florenly
Jurnal Ilmiah Kesehatan Sandi Husada Vol 9 No 1 (2020): Jurnal Ilmiah Kesehatan Sandi Husada
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Akademi Keperawatan Sandi Karsa (Merger) Politeknik Sandi Karsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35816/jiskh.v11i1.313

Abstract

Longan skin (Dimocarpus longan Lour) contains flavonoid compounds, glycosides, saponins, and tannins, so it has an antibacterial capacity. 1% Nanocitosan mixed into plant extracts can increase the inhibitory growth of bacteria in the oral cavity. This study aims to compare the effectiveness of longan skin extract (Dimocarpus longan Lour) added 1% nano chitosan in inhibiting Streptococcusmutans at various concentrations, namely 40%, 60%, and 80%. Longan skin extract is made by maceration method and made with a concentration of 40%, 60%, and 80%. Whereas 1% of nano chitosan is made by the ionic glass method. The two ingredients were mixed in a ratio of 1: 1, and the effectiveness of inhibition was tested by the disk diffusion method. The results showed the mean and standard deviation of longan skin extract at concentrations of 40%, 60% and 80% with 1% nano chitosan was 6.86 ± 0.471 mm; 7.62 ± 0.533 mm and 8.53 ± 0.395 mm. Inhibition with a diameter> 7 mm indicates medium-strength antibacterial. The Shapiro-Wilk test proves that the data are normally distributed. Statistical analysis with one-way Analysis of Variance (ANOVA) showed longan skin extract (Dimocarpus longan Lour) mixed with 1% nano chitosan most effective in inhibiting Streptococcus mutans at 80% concentration, where p = 0,000 (0 <0.05). This is due to an increase in the concentration of phytochemical compounds
Efektivitas Antibakteri Campuran Nanokitosan 1% dengan Berbagai Konsentrasi Ekstrak Biji Kelengkeng Terhadap Staphylococcus Aureus Sabrina Khairunnisa; Titania Aurelya Tandra; Mellisa Sim; Florenly Florenly
Jurnal Ilmiah Kesehatan Sandi Husada Vol 9 No 1 (2020): Jurnal Ilmiah Kesehatan Sandi Husada
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Akademi Keperawatan Sandi Karsa (Merger) Politeknik Sandi Karsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35816/jiskh.v11i1.319

Abstract

As shown on the qualitative phytochemical examinations, longan seeds contain bioactive compounds of flavonoids, glycosides, saponins, and tannins, hence it has antibacterial potential. Whereas nano chitosan, besides having antibacterial activity, is used as a matrix for various types of drugs and plant extracts. This study aims to compare the effectiveness of nano chitosan 1% mix with longan seed extract in inhibiting the growth of Staphylococcus aureus, between 80%, 60%, and 40% concentration. Longan seed extract was prepared using the maceration method and made into various concentrations, i.e. 80%, 60%, and 40%. The nano chitosan 1% was prepared with the ionic gelation method. The two ingredients were mixed in a ratio of 1: 1. The antibacterial investigations on the different concentrations utilized the disk diffusion method. The results presented the mean inhibition power of a mixture of 80% concentration was 9.38 mm; a concentration of 60% (8.47 mm) and a concentration of 40% (7.10 mm). Statistical analysis using one way ANOVA showed the most effective concentration of the mixture in inhibiting Staphylococcus aureus was seen at 80%, where p = 0,000 (p <0.05). The results of this study indicate that increasing the concentration of longan seed extract strengthens the inhibitory power against Staphylococcus aureus. The outcome was justified as the increment in concentration of longan seed extracts lead to an increase in the number of active compounds, thereby upsurge its effectiveness in damaging Staphylococcus aureus cells.
Efek Penambahan Nanokitosan 1% Kedalam Berbagai Konsentrasi Ekstrak Kulit Kelengkeng Streptococcus Mutans Titania Aurely Tandra; Sabrina Khairunissa; Mellisa Sim; Florenly Florenly
Jurnal Ilmiah Kesehatan Sandi Husada Vol 9 No 1 (2020): Jurnal Ilmiah Kesehatan Sandi Husada
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Akademi Keperawatan Sandi Karsa (Merger) Politeknik Sandi Karsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35816/jiskh.v11i1.313

Abstract

Longan skin (Dimocarpus longan Lour) contains flavonoid compounds, glycosides, saponins, and tannins, so it has an antibacterial capacity. 1% Nanocitosan mixed into plant extracts can increase the inhibitory growth of bacteria in the oral cavity. This study aims to compare the effectiveness of longan skin extract (Dimocarpus longan Lour) added 1% nano chitosan in inhibiting Streptococcusmutans at various concentrations, namely 40%, 60%, and 80%. Longan skin extract is made by maceration method and made with a concentration of 40%, 60%, and 80%. Whereas 1% of nano chitosan is made by the ionic glass method. The two ingredients were mixed in a ratio of 1: 1, and the effectiveness of inhibition was tested by the disk diffusion method. The results showed the mean and standard deviation of longan skin extract at concentrations of 40%, 60% and 80% with 1% nano chitosan was 6.86 ± 0.471 mm; 7.62 ± 0.533 mm and 8.53 ± 0.395 mm. Inhibition with a diameter> 7 mm indicates medium-strength antibacterial. The Shapiro-Wilk test proves that the data are normally distributed. Statistical analysis with one-way Analysis of Variance (ANOVA) showed longan skin extract (Dimocarpus longan Lour) mixed with 1% nano chitosan most effective in inhibiting Streptococcus mutans at 80% concentration, where p = 0,000 (0 <0.05). This is due to an increase in the concentration of phytochemical compounds
Efektivitas Antibakteri Campuran Nanokitosan 1% dengan Berbagai Konsentrasi Ekstrak Biji Kelengkeng Terhadap Staphylococcus Aureus Sabrina Khairunnisa; Titania Aurelya Tandra; Mellisa Sim; Florenly Florenly
Jurnal Ilmiah Kesehatan Sandi Husada Vol 9 No 1 (2020): Jurnal Ilmiah Kesehatan Sandi Husada
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Akademi Keperawatan Sandi Karsa (Merger) Politeknik Sandi Karsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35816/jiskh.v11i1.319

Abstract

As shown on the qualitative phytochemical examinations, longan seeds contain bioactive compounds of flavonoids, glycosides, saponins, and tannins, hence it has antibacterial potential. Whereas nano chitosan, besides having antibacterial activity, is used as a matrix for various types of drugs and plant extracts. This study aims to compare the effectiveness of nano chitosan 1% mix with longan seed extract in inhibiting the growth of Staphylococcus aureus, between 80%, 60%, and 40% concentration. Longan seed extract was prepared using the maceration method and made into various concentrations, i.e. 80%, 60%, and 40%. The nano chitosan 1% was prepared with the ionic gelation method. The two ingredients were mixed in a ratio of 1: 1. The antibacterial investigations on the different concentrations utilized the disk diffusion method. The results presented the mean inhibition power of a mixture of 80% concentration was 9.38 mm; a concentration of 60% (8.47 mm) and a concentration of 40% (7.10 mm). Statistical analysis using one way ANOVA showed the most effective concentration of the mixture in inhibiting Staphylococcus aureus was seen at 80%, where p = 0,000 (p <0.05). The results of this study indicate that increasing the concentration of longan seed extract strengthens the inhibitory power against Staphylococcus aureus. The outcome was justified as the increment in concentration of longan seed extracts lead to an increase in the number of active compounds, thereby upsurge its effectiveness in damaging Staphylococcus aureus cells.