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Genetic diversity and haplogroups distributions of Kampung chickens using hypervariable-I mitochondrial DNA control region Arifin Zein, M Syamsul; Sulandari, S
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Until now no studies evaluating the position of Kampung chickens in chicken clade of Asia. Thus studies based on molecular DNA sequence hipervariable-I on Kampung chicken is needed. Molecular studies based on DNA sequences hyper variable-I of Kampong chicken was done to confirm the results of previous evaluations conducted on 15 families of local chickens of Indonesia. An analysis of 210 individuals Kampung chicken (Aceh, North Sumatra, Lampung, Banten, Central Java, Lombok, Sulawesi, Ternate, Morotai and Halmahera) resulted in 51 haplotypes derived from 62 polymorphic sites. Polymorphic sites among the highest seen at 112-397 (93.22%). The highest haplotype frequencies contained in the haplotype H-4 (36.19%), followed by H-1 (18.57%) and H-5 (10, 95%). Kampung chicken phylogeny analysis formed four haplogroups/clade from 7 references of Asian chicken clade. Four haplogroups are clade II = 84.31% (43 haplotypes), clade IIIC = 1.96% (1 haplotype), clade IIID = 3.92% (2 haplotypes), clade IV = 7.84% (4 haplotypes). The results prove of that Indonesian local and indigenous chickens were equally dominated clade II. Analysis of genetic diversity showed haplotype diversity of 0.825 Â± 0.021, nucleotide diversity of 0.00600 on average, the genetic distance between populations ranged from 0.003 to 0.011, and the genetic distance within populations ranged from 0.00395 to 0.01031. Genetic distance between individuals in all populations of Kampung chickens was significantly different (P < 0.01). Fus Fs values was negative, indicating high genetic diversity and population expansion on native chicken in Indonesia. Other important result was shown with the major haplotype spread from western to eastern Indonesia, and had strengthened the position of Indonesia as one of the centers of domestication of the chicken. Key Words: Kampung Chicken, Hypervariable-I, Control Region, Mitochondrial DNA, Haplotype, Clade

Genetic diversity and phylogenetic relationship of Indonesian Local goats using microsatellite DNA markers Syamsul Arifin Zein, M; Sulandari, S; ., Muladno; ., Subandriyo; ., Riwantoro
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

Genetic diversity is important information in the process of conservation and sustainable utilization of animal genetic resources. Thirteen microsatellite markers were used to estimate the degree of genetic diversity on five Indonesian local goats. Results showed the highest average allele diversity present in the locus MAF70 (5.6 Â± 2.9), and the lowest was in the locus MAF035 (1.6 Â± 0.6), the average number of alleles per locus was 6 Â± 2.3. The lowest average alleles diversity present was in the Gembrong goat (2.2 Â± 1.1) and the highest was in the Jawarandu goat (4.9 Â± 2.2). There is a unique alleles at loci MCM527 and present in all Indonesian local goat with the highest allele frequency on Peranakan Etawa (37.2%) and lowest in Gembrong goat (7.9%). H0 ranged from 0.372 Â± 0.173 (Gembrong) to 0.540 Â± 0.204 (Peranakan Etawa), and HE ranging from 0.249 Â± 0.196 (Gembrong) to 0.540 Â± 0.212 (Peranakan Etawa).The genetic differentiation for inbreeding among population (FIS), within population (FIT), and average genetic differention (FST) were 0,0208 (2,08%), 0,1532 (15,32%), and 0,1352 (13,52%), respectively. Locus ILSTS029, BMS1494, MAF035 and INRA0132 had a low PIC value (PIC < 0.25), locus SRCRSP3, OARFCB20, ILSTS005, SPS113, MCM527, and ETH10 provide moderate (PIC = 0.25 to 0.5), and locus CSRD247, MAF70, and ILSTS11 had high value PIC > 0.5. Phylogenetic relationship was consistent with the history of its development based on Kacang goat except was for Gembrong Goat. This research information can be used for conservation strategies and breeding programs on each population of Indonesian local goat. Key Words: Local Goat, Indonesia, Microsatellites, Genetic Diversity, PIC

Analisis D-loop DNA Mitokondria untuk Memposisikan Ayam Hutan Merah dalam Domestikasi Ayam di Indonesia S Sulandari; M.S A Zein
Media Peternakan Vol. 32 No. 1 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.68 KB)

The current poultry is a domesticated chickens used for both meat and egg production. Pedigree investigation is an important part to understand the process of chicken domestication in Indonesia. Molecular DNA approach using D-loop Mitochondrial DNA marker (hypervariable 1 segment) was used in this analysis. The objective of the study was to construct the pedigree analysis of Indonesian chicken. Four hundreds and eighty four (434) samples belonging to 15 breeds of Indonesian local chicken (Cemani, Kedu, Kedu Putih, Pelung, Sentul, Wareng, Merawang, Kapas, Kate, Arab Silver, Arab Gold, Gaok, Nunukan, Kalosi and Tolaki) and 9 samples of Red jungle fowls (Gallus gallus gallus) were extracted, PCR amplified and subsequently sequenced. Four sequence references from GeneBank, Gallus gallus (NCBI, accession number AB0986688). G. gallus (GenBank accession number AB098668), G. gallus spadiceus (GenBank accession number AB007721), and G. gallus bankiva (GenBank accession number AB007718) were included in this analysis. The sequences of the first 397 nucleotides were used for analysis. The results show that 72 haplotypes were identified from 56 polymorphic sites. Phylogenetic analysis showed that Indonesian chicken have a close relationship with 2 subspecies of Gallus gallus (G. gallus gallus and G. gallus spadiceus). Our results suggest that D-loop region is highly variable in Indonesian chicken with large number of haplotypes. Key words: Gallus gallus, Indonesian chicken, D-loop Mitochondrial DNA

Genetic diversity and haplogroups distributions of Kampung chickens using hypervariable-I mitochondrial DNA control region M Syamsul Arifin Zein; S Sulandari
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Until now no studies evaluating the position of Kampung chickens in chicken clade of Asia. Thus studies based on molecular DNA sequence hipervariable-I on Kampung chicken is needed. Molecular studies based on DNA sequences hyper variable-I of Kampong chicken was done to confirm the results of previous evaluations conducted on 15 families of local chickens of Indonesia. An analysis of 210 individuals Kampung chicken (Aceh, North Sumatra, Lampung, Banten, Central Java, Lombok, Sulawesi, Ternate, Morotai and Halmahera) resulted in 51 haplotypes derived from 62 polymorphic sites. Polymorphic sites among the highest seen at 112-397 (93.22%). The highest haplotype frequencies contained in the haplotype H-4 (36.19%), followed by H-1 (18.57%) and H-5 (10, 95%). Kampung chicken phylogeny analysis formed four haplogroups/clade from 7 references of Asian chicken clade. Four haplogroups are clade II = 84.31% (43 haplotypes), clade IIIC = 1.96% (1 haplotype), clade IIID = 3.92% (2 haplotypes), clade IV = 7.84% (4 haplotypes). The results prove of that Indonesian local and indigenous chickens were equally dominated clade II. Analysis of genetic diversity showed haplotype diversity of 0.825 ± 0.021, nucleotide diversity of 0.00600 on average, the genetic distance between populations ranged from 0.003 to 0.011, and the genetic distance within populations ranged from 0.00395 to 0.01031. Genetic distance between individuals in all populations of Kampung chickens was significantly different (P < 0.01). Fu's Fs values was negative, indicating high genetic diversity and population expansion on native chicken in Indonesia. Other important result was shown with the major haplotype spread from western to eastern Indonesia, and had strengthened the position of Indonesia as one of the centers of domestication of the chicken. Key Words: Kampung Chicken, Hypervariable-I, Control Region, Mitochondrial DNA, Haplotype, Clade

Genetic diversity and phylogenetic relationship of Indonesian Local goats using microsatellite DNA markers M Syamsul Arifin Zein; S Sulandari; Muladno .; Subandriyo .; Riwantoro .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Genetic diversity is important information in the process of conservation and sustainable utilization of animal genetic resources. Thirteen microsatellite markers were used to estimate the degree of genetic diversity on five Indonesian local goats. Results showed the highest average allele diversity present in the locus MAF70 (5.6 ± 2.9), and the lowest was in the locus MAF035 (1.6 ± 0.6), the average number of alleles per locus was 6 ± 2.3. The lowest average alleles diversity present was in the Gembrong goat (2.2 ± 1.1) and the highest was in the Jawarandu goat (4.9 ± 2.2). There is a unique alleles at loci MCM527 and present in all Indonesian local goat with the highest allele frequency on Peranakan Etawa (37.2%) and lowest in Gembrong goat (7.9%). H0 ranged from 0.372 ± 0.173 (Gembrong) to 0.540 ± 0.204 (Peranakan Etawa), and HE ranging from 0.249 ± 0.196 (Gembrong) to 0.540 ± 0.212 (Peranakan Etawa).The genetic differentiation for inbreeding among population (FIS), within population (FIT), and average genetic differention (FST) were 0,0208 (2,08%), 0,1532 (15,32%), and 0,1352 (13,52%), respectively. Locus ILSTS029, BMS1494, MAF035 and INRA0132 had a low PIC value (PIC < 0.25), locus SRCRSP3, OARFCB20, ILSTS005, SPS113, MCM527, and ETH10 provide moderate (PIC = 0.25 to 0.5), and locus CSRD247, MAF70, and ILSTS11 had high value PIC > 0.5. Phylogenetic relationship was consistent with the history of its development based on Kacang goat except was for Gembrong Goat. This research information can be used for conservation strategies and breeding programs on each population of Indonesian local goat. Key Words: Local Goat, Indonesia, Microsatellites, Genetic Diversity, PIC

PENDAPATAN ASLI DAERAH, BELANJA PEMERINTAH, DAN INDEKS PEMBANGUNAN MANUSIA DALAM MEMPENGARUHI PDRB KABUPATEN SUMBAWA Sulandari, S; Sucihati, Roos Nana
Jurnal Ekonomi & Bisnis Vol. 13 No. 1 (2025): Jurnal Ekonomi & Bisnis
Publisher : Pusat Riset dan Publikasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58406/jeb.v13i1.2079

This study aims to determine the effect of Local Revenue, Government Expenditure, and Human Development Index on GRDP of Sumbawa District. The type of this study was associative study with quantitative approach. The type of data used was quantitative obtained from secondary sources using documentation techniques. The data used in this study are data on Local Revenue, Government Expenditure, and Human Development Index, and GRDP of Sumbawa District for 2014-2023. Data were analyzed using multiple linear regression analysis techniques, partial hypothesis testing (t test), simultaneous hypothesis testing (F test), and coefficient of determination testing (). The result of this study showed that the Local Revenue, Government Expenditure, and Human Development Index had a positive and significant effect on GRDP of Sumbawa District for 2014-2023, either partially or simultaneously. The ability of the Local Revenue, Government Expenditure, and Human Development Index variables to effected on GRDP of Sumbawa District was 94.3%, while the remaining of 5.7% was effected by other variables outside this research model, such as population, workforce, foreign investment and domestic investment.

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