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All Journal Indonesia Medicus Veterinus Jurnal Medik Veteriner
Ketut ELI Supartika
Udayana University
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Veterinary

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Perbandingan Sensitivitas dan Spesifisitas Uji Pewarnaan Sellers’ dan Fluorescent Antibody Technique (FAT) dalam Mendiagnosa Penyakit Rabies pada Anjing di Bali BOGIA, STEVEN YOHANES; Kardena, I Made; Supartika, Ketut ELI
Indonesia Medicus Veterinus Vol.1 (1) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.659 KB)

Abstract

Telah dilakukan penelitian terhadap 109 sampel otak anjing yang dicurigai terinfeksi rabies dari berbagai wilayah di Bali. Penelitian ini bertujuan untuk membandingkan tingkat sensitivitas dan spesifisitas dari uji Seller’s dan Fluorescent Antibody Technique (FAT) dalam mendiagnosa penyakit rabies pada anjing di Bali. Hasil uji seluruh sampel baik uji Sellers’ maupun FAT ditabulasi, lalu dianalisi dengan tabel 2 X 2. Hasil menunjukkan uji Sellers’ yang digunakan dalam mendiagnosa penyakit rabies pada anjing memiliki tingkat sensitivitas sebesar 64,10% dan spesifisitas sebesar 98,57%. Namun, uji FAT didapatkan tingkat sensitivitas sebesar 97,43% dan spesifisitasnya sebesar 100%. Tingkat sensitivitas dan spesifisitas dari uji FAT yang lebih besar dibandingkan dengan tingkat sensitifitas dan spesifisitas dari uji Sellers’ yang digunakan untuk mendiagnosa penyakit rabies pada anjing di Bali menunjukkan validitas dari uji FAT lebih baik dibandingkan uji Seller’s.
Indeks Mitosis Venereal Sarcoma pada Anjing Lokal di Denpasar Nasrudin, Fathurohman; Kardena, Made; Supartika, Ketut Eli
Indonesia Medicus Veterinus Vol 3 (4) 2014
Publisher : Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui rataan indeks mitosis serta perbedaan rataan indeks mitosis kasus venereal sarcoma pada anjing lokal jantan dan betina di Denpasar. Data epidemiologi dan pemeriksaan histopatologi dari total 42 sampel anjing lokal penderita venereal sarcoma diperoleh dari Balai Besar Veteriner Denpasar, yang terdiri dari 21 sampel anjing lokal jantan dan 21 sampel anjing lokal betina. Indeks mitosis dihitung dengan menentukan rataan hitung dari figur mitotis pada masing-masing sampel organ pada setiap empat lapang pandang. Hasil pemeriksaan 42 sampel anjing lokal penderita venereal sarcoma di Denpasar didapatkan jumlah rataan indeks mitosis venereal sarcoma yakni sebanyak 6,83 indeks mitosis. Hasil rerata indeks mitosis venereal sarcoma pada anjing lokal jantan dan betina di Denpasar, masing-masing sebesar 7,619 dan 6,06 (p>0,05). Kedua hasil tidak berbeda secara nyata, hal ini mengindikasikan venereal sarcoma pada anjing lokal jantan dan betina di Denpasar memiliki tingkat keganasan yang sama.
Pathogenesis studies of African swine fever virus isolates from Bali and East Nusa Tenggara as a basis for inactivated vaccine development Tenaya, I Wayan Masa; Agustina, Kadek Karang; Suada, Ketut; Sukada, Made; Mufa, Romy Muhammad Dary; Apsari, Ida Ayu Pasti; Dwinata, I Made; Ardana, Ida Bagus Komang; Damriyasa, Made; Sari, Tri Komala; Astawa, Nyoman Mantik; Supartika, Ketut Eli; Wirata, Ketut; Suarsana, Nyoman; Suartha, Nyoman
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.150-160

Abstract

African swine fever (ASF) is a highly contagious and lethal disease of pigs for which no effective vaccine is currently available. This study evaluated the pathogenicity of field African swine fever virus (ASFV) isolates from Bali and East Nusa Tenggara and assessed their potential for inactivated vaccine development using spleen-derived antigens. Based on the qPCR results, among all ASFV isolates tested, only the Bali isolate, designated B1, exhibited the highest DNA concentration, suggesting that this sample contained the highest ASFV titer. Consequently, the B1 isolate was selected and processed into chemically treated (CT-ASFV) and non-treated (NCT-ASFV) formulations. In the pathogenesis trials, pigs inoculated with NCT-ASFV developed acute ASF and died within 12 days, whereas those receiving CT-ASFV exhibited no clinical signs of ASF and showed no evidence of viral replication. In the vaccination trial, pigs immunized with CT-ASFV emulsified in Montanide™ ISA 50 V2 demonstrated survival in two out of three animals (67%) following challenge with a virulent strain, while all control animals succumbed to infection. Despite the absence of detectable humoral responses as determined by ELISA, the observed protection suggests a potential role for cell-mediated immunity. These findings indicate that the B1 isolate was highly virulent and represents a promising candidate for the development of an inactivated ASF vaccine. Further evaluation in larger-scale field trials is warranted.
Co-Authors Fathurohman Nasrudin I Made Dwinata I Made Kardena I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Wayan Masa Tenaya, I Wayan Masa Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Kadek Karang Agustina KETUT SUADA Made Damriyasa, Made Mufa, Romy Muhammad Dary Nyoman Suartha STEVEN YOHANES BOGIA Sukada, Made TRI KOMALA SARI Wirata, Ketut