Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Tropical Animal Science Journal
T. G. Wandita
Department of Animal Life and Environmental Science, Hankyong National University 327 Jungang-ro, Anseong-si, Gyeonggi-do 456-749 South Korea
Agriculture, Biological Sciences & Forestry Energy

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Pre-Adipocyte Determination and Adipocyte Differentiation of Stromal Vascular Cells Isolated From Intramuscular Tissue of Hanwoo Beef Cattle Treated by Acetate and Propionate T. G. Wandita; N. Joshi; H. H. Kim; S. J. An; S. G. Hwang
Tropical Animal Science Journal Vol. 41 No. 3 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (980.522 KB) | DOI: 10.5398/tasj.2018.41.3.207

Abstract

The effects of acetate and propionate in enhancing intramuscular fat (marbling) deposition in beef cattle are poorly understood. This study aimed to evaluate the effects of acetate and propionate on pre-adipocyte determination and adipocyte differentiation in stromal vascular cells (SVC) isolated from the intramuscular tissue of Hanwoo beef cattle. SVC were isolated and treated with different concentrations of acetate and propionate (0.125 to 1 mM). Pre-adipocyte determination was observed through the Zfp423 (Zinc finger protein 423) and Pref-1 (Pre-adipocyte factor-1) genes expressions, which were important transcription factors used to identify committed pre-adipocytes. Adipocyte differentiation was determined based on the lipid accumulation, triglyceride contents, and their molecular activities related to adipogenic markers. The results suggest that acetate supports pre-adipocyte determination in SVC which is observed to increase the expression of Zfp423 and Pref-1 along with the increasing acetate concentration. Propionate treatment was seen to significantly affect lipid accumulation as well as triglyceride content in SVC, compared to acetate treatment. Furthermore, propionate treatment very significantly increased the expressions of PPARγ, C/EBPα, SREBP-1c, and FABP4. In conclusion, acetate likely enhances pre-adipocyte determination, while propionate effectively promotes adipogenesis in SVC isolated from the intramuscular tissue of Hanwoo beef cattle.
Co-Authors H. H. Kim N. Joshi S. G. Hwang S. J. An