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All Journal Tropical Animal Science Journal
R. I. Arifiantini
Department of Veterinary Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University
Agriculture, Biological Sciences & Forestry Energy

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Characteristics and Potential Production of Frozen Semen of Pasundan Bull Santoso Santoso; Herdis Herdis; R. I. Arifiantini; A. Gunawan; C. Sumantri
Tropical Animal Science Journal Vol. 44 No. 1 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.1.24

Abstract

Pasundan cattle are one of the Indonesian domestic resources of animal-genetic materials that need to be developed and conserved. The aims of this study were to evaluate the characteristics of sperm and the potential production of frozen semen of Pasundan bulls. Ten Pasundan bulls were divided into two groups based on their fresh sperm motilities. Pasundan bulls were grouped based on their sperm motilities into group A (70-79%) and group B (80-89%). Secondary data were collected and confirmed with the primary data. The semen volume, pH, color, consistency, mass movement, sperm concentration, motility, viability, membrane integrity, abnormality, and DNA integrity of fresh semen were evaluated. The semen was then diluted, frozen, and stored at -196°C. The results showed that the pH of the fresh semen in group B was lower (p<0.05) than group A. Sperm concentration per ejaculate showed no difference with a range of 4312.36x106 to 6303.52x106. The viability and DNA integrity of fresh semen were not different between group A (84.41±0.99%; 91.19±0.79%) and group B (86.35±2.16%; 92.58±0.35%). DNA integrity of frozen semen of group B (89.81±1.18%) was higher (p<0.05) than that of group A (86.83±0.60%). The sperm motility of frozen semen of group A (<40%) was lower compared to SNI number 4869-1:2017. The frozen semen production of Pasundan bulls was between 144.18 to 191.29 straws/ejaculate. In conclusion, only bulls from fresh-semen sperm motility 80%-89% (group B) are eligible to be used as a semen source for artificial insemination.
The Quality of Frozen Semen of Limousin Bull in Various Semen Diluents A. A. Arif; T. Maulana; E. M. Kaiin; B. Purwantara; R. I. Arifiantini
Tropical Animal Science Journal Vol. 45 No. 3 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.3.284

Abstract

Diluents are substances added to the semen to increase the semen volume, reduce sperm density, and maintain sperm viability. This study aimed to compare the effects of various semen diluents on the quality of frozen semen of Limousin bull. Three mature Limousin bulls were used as semen sources. The semen was collected using an artificial vagina and then evaluated for its quality. Sperms with motility less than 70% and abnormality greater than 20% were excluded from this study. After semen evaluation, each ejaculate was individually divided into four equal tubes and diluted with skimmed milk-egg yolk (SMEY), Tris–egg yolk (TEY), Tris–egg yolk–skimmed milk (TEYSM), or Andromed®. Diluted semen was loaded into a mini straw and then equilibrated at 5 °C for 4 h. Following equilibration, the straws were frozen. The percentages of sperm motility, viability, intact membrane, and sperm DNA integrity were evaluated after freezing. Furthermore, malondialdehyde (MDA) and aspartate aminotransferase (AST) enzyme concentrations were assessed after thawing. The sperm motility with TEY, TEYSM, and Andromed® did not differ significantly but was higher than with SMEY. Sperm viability, membrane intactness, and sperm DNA integrity did not differ among treatments. By contrast, the MDA levels and concentration of AST in the Andromed® diluent semen showed the lowest values compared with SMEY semen (p<0.05) but did not differ from those of TEY and TEYSM semen. To conclude, TEY, TEYSM, and Andromed® diluents performed equally well, and although they showed better results than SMEY, sperm diluted in SMEY are still suitable for artificial insemination.
Co-Authors A. A. Arif A. Gunawan B. Purwantara C. Sumantri E. M. Kaiin Herdis Herdis Santoso Santoso T. Maulana