L. Krismiyanto
Faculty of Animal and Agricultural Sciences, Diponegoro University

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Immune Status and Growth of Broiler Fed Diet with Microparticle Protein Added with Natural Acidifier N. Suthama; B. Sukamto; I. Mangisah; L. Krismiyanto
Tropical Animal Science Journal Vol. 44 No. 2 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.2.198

Abstract

The present study was conducted to evaluate the feeding effect of diet composed of microparticle protein added with lime juice as an acidifier on immune status and efficiency of protein utilization in relation to the growth of broiler chicken. Dietary treatments tested were P1 was diet with 20% intact protein and CaCO3 as a Ca source, P2 was diet with 20% microparticle protein and eggshell as a Ca source added with 1.2% lime juice, and P3 was diet with 20% microparticle protein and eggshell as a Ca source added with 2.4% lime juice. Variables measured were protein and amino acids digestibility, retentions of N and Ca, fecal Nτ–methyl histidine (Nτ–MH) excretion as a protein turnover index, lymphoid organs, heterophil and lymphocyte (H/L) ratio, immunoglobulin A (IgA) as a body resistance indicator, feed consumption, body weight gain (BWG), and feed conversion ratio (FCR). The experiment was arranged in a completely randomized design with 3 treatments and 8 replications. Data were subjected to analysis of variance and Duncan test at 5% probability. Protein and amino acid digestibility, N, and Ca retentions were significantly (p<0.05) higher in P2 and P3. However, the H/L ratio was significantly (p<0.05) lower in both treatments compared to P1. Bursa fabricius and lymph weights were not difference, but BWG in P2 significantly (p<0.05) increased. In conclusion, protein and some essential amino acids digestibility, N and Ca retentions, IgA, and BWG increase with the decrease in H/L ratio, fecal Nτ–MH, feed consumption, and FCR in broiler fed diet composed of microparticle protein source with additional 1.2 mL acidifier of lime juice.
Antioxidant Enzymes and Growth of Broiler Fed Microparticle Protein Diet with Inulin or Lactobacillus acidophillus Supplementation N. Suthama; B. Sukamto; I. Mangisah; L. Krismiyanto
Tropical Animal Science Journal Vol. 46 No. 1 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.1.97

Abstract

The effect of dahlia tuber extract or Lactobacillus acidophilus inclusion on intestinal physiology, antioxidant enzymes, and growth performance of broiler chicken given a microparticle protein-composed diet was evaluated in the present study. Three dietary treatments were applied, Control: 21% intact protein diet without additive, MP-DTE: 21% microparticle protein diet + 1.2% dahlia tuber extract as inulin source, and MP-La: 21% microparticle protein diet + 1.2 mL L. acidophilus (1 mL L. acidophilus/La equal to 108 cfu). Microparticle proteins were obtained from common protein source ingredients for poultry, fish meal, and soybean meal. A completely randomized design was assigned with 3 treatments and replicated 8 times, 10 broilers in each replication. Experimental animals were 240 broilers for treatment and 10 birds for endogenous correction. Digestibility of protein and essential amino acids, villi height, intestinal bacterial counts (LAB and Eschericia coli), short chain fatty acids/SCFA (acetate, propionate, and butyrate), antioxidant enzymes (GSH-Px and SOD), and growth performances (meat protein/MPM and fat mass/MFM, feed consumption, body weight gain/BWG, and feed conversion ratio/FCR) were variables measured. Data were statistically processed based on analysis of variance and continued to the Duncan test (p<0.05). Supplementation of dahlia inulin extract or L. acidophilus to the micropaticle protein diet significantly (p<0.05) increased N retention, villi height, LAB population, SCFA, antioxidant enzymes, and improved MPM and BWG, but decreased E. coli count, MFM, and FCR. However, feed consumption was not affected by any treatment. It can be concluded that L. acidophilus supplementation to the microparticle protein diet (MP-La) improves antioxidant enzymes, and growth performance with high meat protein and low fat mass.