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All Journal Tropical Animal Science Journal
D. Lestari
Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Agriculture, Biological Sciences & Forestry Energy

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Polymorphism and Association of DMA Gene with Total IgY Concentration and ND Antibody Titer in IPB-D2 Chicken Line D. Lestari; S. Murtini; N. Ulupi; C. Sumantri
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.1

Abstract

The DMA (DM α chain) gene, a member of non-classical MHC class II plays an important role in the process of presenting antigen peptides by producing DM protein. This study aimed to identify the polymorphism of DMA gene and their associations with disease resistance traits such as total IgY concentration and ND antibody titers in IPB-D2 chicken line. The total sample used was 101 chickens consisted of 81 IPB-D2 G0 chickens (53 female and 28 male) and 20 SENSI-1 chickens (15 female and 5 male). Blood samples were collected at 21 weeks old for genotyping, total IgY concentration analysis, and ND antibody titer analysis. SENSI-1 chickens were used as a comparison in the analysis of DMA gene polymorphism. The method for DNA polymorphism of DMA gene was direct-DNA sequencing. The total IgY concentration was analyzed using the indirect ELISA method, while the ND antibody titer used the HI test. Data were analyzed using GLM, genotypic, and haplotype mean values compared with t-test. The results showed that 4 SNPs were found, i.e., g.2328 G>A (exon 3), g.2503 A>G (intron 3), g.2612 G>A, and g.2686 G>A (exon 4). The SNPs of DMA gene were found polymorphic with three genotypes (GG, AG, and GG), and the expected SNP g.2503 A>G only had two genotypes (AA and GG). The g.2328 G>A was associated (p<0.05) with total IgY concentration. A combination of 4 SNPs formed 7 haplotypes. Haplotype 1 was associated with total IgY concentration (p<0.05), and haplotype 5 was associated with ND antibody titer (p<0.05). In conclusion, the g.2328 G>A mutation and haplotype 1 could be potentially recommended as a genetic marker for high total IgY concentration, and haplotype 5 could be potentially recommended as a genetic marker for ND antibody titer in IPB-D2 chicken line.
Variation and Association of Avian β-Defensin 2 Gene with the Concentration of Immunoglobulin Y and the Titer of Newcastle-Disease Antibody in IPB-D1 Chicken D. Lestari; Masruroh; I. Khaerunnisa; S. Murtini; N. Ulupi; A. Gunawan; C. Sumantri
Tropical Animal Science Journal Vol. 45 No. 2 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.2.121

Abstract

Defensins play roles in innate immunity by exhibiting antimicrobial activity against microbes such as gram-negative and -positive bacteria, viruses, and fungi. This study aimed to identify variants of the Avian β-Defensin 2 (AvBD2) and determine their associations with the concentration of immunoglobulin Y and the titer of Newcastle disease (ND) antibody in IPB-1 chicken. The chicken population used in this study was 21-week-old IPB-D1 chickens (n=90). Variations in AvBD2 were analyzed by direct DNA sequencing. IgY concentration was measured by indirect ELISA, and the titer of ND antibody was measured by the hemagglutination-inhibition test. The AvBD2 association was analyzed by the general linear model procedure and Duncan’s multiple range test. The results revealed 10 SNPs located in intron 1 (3 SNPs), exon 2 (3 SNPs), and intron 2 (4 SNPs). Six of these SNPs were associated with IgY concentration. The CC genotype of g.5002 C>T was associated with IgY concentration and produced the highest mean IgY concentration. This g.5002 C>T mutation results in alanine-to-valine substitutions. The CC genotype of g.5002 C>T could be considered as a criterion for selecting chickens with high IgY concentrations.
Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index D. Lestari; S. Murtini; N. Ulupi; A. Gunawan; C. Sumantri
Tropical Animal Science Journal Vol. 46 No. 3 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.3.287

Abstract

The CD4 gene plays an important role in the immune process by producing CD4 molecules that aid in producing antibodies. IPB-D2 chickens are selected from IPB-D2 chickens based on IgY concentration and ND antibody titer. This study aimed to analyze the polymorphism of the CD4 gene, unravel the mRNA expression of CD4 gene in IPB-D2 chicken related to the immunocompetence index, and detect the CD4 gene pathway. The total samples used were 100 IPB-D2 G2 chickens aged 21 weeks. Blood samples were collected for ELISA test, HI test, sequencing test, and seca tonsil tissue for relative mRNA expression. Polymorphism and association data were analyzed using MEGAX, FinchTV, SNPstat, and DNAsp. The relative mRNA expression analysis was conducted using qRT-PCR. The pathway analysis of the CD4 gene was performed using the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis. The result showed there were 4 SNPs of the CD4 gene in IPB-D2 chicken, i.e., g.7526 C>T, g.7825 C>T, g.8100 C>A, and g.8157 T>A. All CD4 SNPSs showed no association with IgY concentration and ND antibody titers. Relative mRNA expression shows that IPB-D2 chickens with high ND antibody titers have a higher level of expression when compared to IPB-D2 chickens with low ND antibody titers. Furthermore, pathway analysis showed the CD4 gene involved in the T cell receptor (TcR) signaling process. This study concludes that the CD4 gene is polymorphic and involved in the T cell receptor signaling process. This study demonstrated that polymorphisms of the CD4 gene in IPB-D2 chicken might not contribute to the IgY concentration and ND antibody titer but can serve as a reference in the study of CD4 genes in the other chicken breeds related to the other immunocompetence index.
Co-Authors A. Gunawan C. Sumantri I. Khaerunnisa Masruroh N. Ulupi S. Murtini S. Murtini