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M. M. Rahman
Faculty of Agro Based Industry, Universiti Malaysia Kelantan
Agriculture, Biological Sciences & Forestry Energy

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Journal : Tropical Animal Science Journal

 1 
Somatic Embryogenesis and Plant Regeneration from the Apical Meristem of Wrukwona Napiergrass (Pennisetum purpureum) Treated with Thidiozuron and Cupric Sulfate N. Umami; A. N. Respati; M. M. Rahman; K. Umpuch; T. Gondo
Tropical Animal Science Journal Vol. 45 No. 2 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.2.220

Abstract

This study focused on the effectiveness of somatic embryogenesis and regenerated plant in Wrukwona napiergrass. Previously, we studied in vitro propagation of 4 cultivars of napiergrass (Pennisetum purpureum) and showed that only 3.3% of Wrukwona cultivar formed embryogenic callus on day 30 and 21.7% on day 60 of incubation. To improve callus formation performance, it is necessary to develop a special propagation method for Wrukwona cultivar in terms of various growth regulators and additional compounds. This study used several rates of 2.4-dichlorophenoxyacetic acid (2.4-D), benzyl amino purine (BAP), and thidiozuron (TDZ). The result showed that the use of medium Murashige & Skoog (MS) with 2.4-D and BAP at a high ratio of 2.4-D, and TDZ 2 µM formed 78.6% embryogenic callus on day 60th and no albino was found in the regenerated plant. The best combination of growth promotor for embryogenic callus formation was 3 mg L-1 2.4-D, 0.5 mg L-1 BAP, and 2 µM TDZ. Callus proliferation with MS media added with 3 mg L-1 2.4-D, 0.5 mg L-1 BAP, 2 µM TDZ, and 5 µM CuSO4 gave the best proliferation results, with regeneration reaching 65%. All regenerants successfully grew in soil. It can be concluded that somatic embryogenesis of P. purpureum cv. Wrukwona can be produced from MS culture medium using 2 mg L-1 2.4-D, 0.5 mg L-1 BAP, and 2 µM TDZ. Effective multiplication was carried out by adding 5 µM CuSO4 to the same medium as the embryogenic callus formation, and effective regeneration was carried out with MS media containing 2 mg L-1 BAP.
Selenium Application in Improving Chicory (Cichorium intybus) Productivity and Quality N. Umami; E. R. V. Rahayu; B. Suhartanto; A. Agus; M. M. Rahman
Tropical Animal Science Journal Vol. 45 No. 3 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.3.337

Abstract

Selenium (Se) is an essential mineral element needed by livestock and human. The amount of Se intake is largely determined by the Se content in the plants (food and feed) consumed. This study aimed to analyze the effect of Se fertilizer on the morphological characters, biomass yield, nutrient content, and in vitro nutrient digestibility of Cichorium intybus. This study was conducted for four months, starting from May to August 2019, located at the Faculty of Animal Science, Universitas Gadjah Mada. The seeds of Cichorium intybus var. Chico was sown in 24 plots and the plots were arranged in a completely randomized block design. Three levels of Se fertilization treatments (0, 3.5, and 7.5 mg/m2) were applied with 8 replicates. Plant defoliation on every 45 days: namely the first defoliation (from sowing to 45 days), the second defoliation (regrowth 1 up to 45 days), and the third defoliation (regrowth 2 up to 45 days). In all studied parameters, the results showed that chicory with Se fertilizer (3.5 and 7.5 mg/m2) was higher (p<0.05) than chicory without Se fertilization. The chicory with Se fertilizer at the level of 7.5 mg/m2 had higher (p<0.05) leaf width, biomass yield, crude protein, and Se content, and in vitro nutrient digestibility (dry matter, organic matter, crude protein) compared to that with Se fertilizer at the level of 3.5 mg/m2. Chicory at the third defoliation had a higher biomass yield and Se content than at the second defoliation. In conclusion, the best combination from this study was the third defoliation, with Se fertilizer level of 7.5 mg/m2.
Co-Authors A. Agus A. N. Respati B. Suhartanto E. R. V. Rahayu K. Umpuch N. Umami N. Umami T. Gondo