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PENGARUH PENAMBAHAN MnSO4 TERHADAP AKTIVITAS ENZIM MANGAN PEROKSIDASE PADA DELIGNIFIKASI LIMBAH BAGASSE OLEH PHLEBIA SP. MG-60 Nurika, Irnia; Majid, Zuliyan Agus Nur Muchlis; Suprayogi, -
Jurnal Teknologi Pertanian Vol 20, No 3 (2019)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.144 KB) | DOI: 10.21776/ub.jtp.2019.020.03.3

Abstract

ABSTRAKKandungan lignin pada bagasse dapat dimanfaatkan sebagai bahan kimia bernilai tinggi melalui proses degradasi lignoselulosa. Phlebia sp. MG-60 merupakan salah satu jenis jamur pelapuk yang menghasilkan enzim mangan peroksidase (MnP) untuk mendegradasi lignin. Akan tetapi, untuk meningkatkan aktivitas enzim MnP dibutuhkan induser seperti MnSO4. Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan MnSO4 terhadap aktivitas enzim MnP Phlebia sp. MG-60 dalam  proses delignifikasi selama 28 hari inkubasi. Mangan dengan konsentrasi 0 mM; 0,1 mM; dan 0.5 mM ditambahkan pada substrat yang telah diinokulasi dengan Phlebia sp. MG-60 dan diinkubasi selama 28 hari. Hasil kemudian diekstrak selanjutnya diuji kadar lignin dan aktivitas enzim MnP yang dihasilkan menggunakan spektofotometri. Hasil menunjukkan aktivitas enzim MnP bagasse yang paling besar dengan penambahan 0,1 mM MnSO4 0.605 IU/mL dengan lignin removal mencapai 31.60%.  ABSTRACTLignin compound in bagasse can be used as a high-value chemical through lignocellulose degradation. Phlebia sp. MG-60 is one type of rot fungus that produces the MnP enzyme to degrade lignin. However, to increase the MnP activity, inducer such as MnSO4 is needed. The purpose of this study was to determine the effect of MnSO4 on Phlebia sp. MG-60 performance and MnP activity of Phlebia sp. MG-60 during 28 days degradation of lignocellulose. Manganese with a concentration of 0 mM; 0,1 mM; and 0.5 mM was added to the substrate which had been inoculated with Phlebia sp. MG-60 and incubated for 28 days. The results were then extracted furthermore lignin content assay and MnP enzyme activity tested by using spectrophotometry. The results showed the greatest bagasse MnP enzyme activity with the addition of 0,1 mM MnSO4 0.605 IU / mL with lignin removal reached of 31.60%.
PENYELESAIAN SENGKETA PERDATA DI LUAR SIDANG PENGADILAN (STUDI KASUS PENGUSAHA CATERING DI KOTA SEMARANG) Suprayogi, -
Forum Ilmu Sosial Vol 35, No 1 (2008): June 2008
Publisher : Faculty of Social Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/fis.v35i1.1296

Abstract

The Existence of law in the society is badly needed to keep the orderliness, safety and peace. Besides,law is also used to settle the conflicts within the society. Some conflicts belong to the civil law occursinacontract law, in which there is an agreement inside. The agreement which made by the involved partsof dispute settlement. If there is a dispute, it will be settled trough the court or outside the court. Theproblem of theresearch is stated as follows: what factors which make the people prefer to settles their civildispute outside the court? The problem is divided into: (1) What reasons which make the civil dispute inthe society of Semarang Municipality? (2) What factors which make the settlement of civil dispute doneoutside the court? ; What is the effect of that kind of settlement? The aims of the research are: (1) to knowthe reasons of emerging the civil dispute in Semarang Municipality; (2) to know the factors which makethe people settle their civil dispute outside the court. The result shows that the factor, which influencesthe catering businessmen/women in settling their dispute outside the court, is motivated by cultural andeconomic factors. Based on the result, it is suggested to settle the dispute outside the court firstly, to getthe intended settlement live, all parts involved in the dispute get the satisfaction and also fulfil their needs.Keywords: Settlement of civil dispute, outside the court, catering business
Co-Authors Irnia Nurika Majid, Zuliyan Agus Nur Muchlis