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All Journal JPSCR : Journal of Pharmaceutical Science and Clinical Research
Anif Nur Artanti
Pharmacy Department, Sebelas Maret University
Environmental Science Public Health

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POTENCY OF RADICAL SCAVENGING ACTIVITY FROM ETHYL ACETATE FRACTION OF Phaseolus vulgaris L., N. lappaceum L., and Pleurotus ostreatus Anif Nur Artanti; Nestri Handayani; Nisya Arienda; Rika Triyoga Prasetyo Rini; Astrid Octaviani
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 1, No 1 (2016)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.494 KB) | DOI: 10.20961/jpscr.v1i1.698

Abstract

Several studied shows that some plants and part of them could protect human body from oxidation of lipid and free radical danger exposure. Various possibilities can occur as a result of the free radical danger exposure, including malfunctioning of cells, damage cell structures, until the occurrence of cancer. One of the compounds found effication as an antioxidant is flavonoid. This study has been done to examine ethyl ascetate fraction from Phaseolus vulgaris L., N. lappaceum L., andPleurotus ostreatus which has potency as radical scavenger. Antiradical activity assay was determined by DPPH method (1,1 difenil-2-pikrilhidrazil). Based on the study the radical scavenging activity respectively from the lowest to the highest activity are vitamin C (5,4 µg/ml) ; fraction from Nephelium lappaceum L (21,81µg/ml) ; fraction from Phaseolus vulgaris L (50,64µg/ml) and Pleurotus ostreatus(80,51µg/ml).
PERBEDAAN KADAR KAFEIN DAUN TEH (Camellia sinensis (L.) Kuntze) BERDASARKAN STATUS KETINGGIAN TEMPAT TANAM DENGAN METODE HPLC Anif Nur Artanti; Wahyu Rohmatin Nikmah; Discus Hendra Setiawan; Fea Prihapsara
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 1, No 1 (2016)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.731 KB) | DOI: 10.20961/jpscr.v1i1.690

Abstract

Tea is the most widely consumed beverage in the world next to water. Tea leaves contain caffeine compound that has many advantages for health. Generally tea garden is in hill, especially in the mountain area which has difference high place that can influence the secondary metabolic compound in plant. So, it needs to observe about the effect of difference height planting place to tea leaf caffeine contain. This research was experimental research that taken samples from tea leaf TRI 2024 variety from 3 height variations, is 800 m; 1000 m; and 1200 m above sea level. The analytical method used in this research is high performance liquid chromatography (HPLC). The HPLC instrument is Lab Alliance brand, LC-10AT VP pump, coulomb ACE 5 C18 and UV-Vis detector at 275 nm length.  The difference of caffeine content was analyzed using ANOVA.The result showed that any influence between height planting and caffeine content. Caffeine content in height 800 m above sea level is 562,20782 mg/gram sample, in height 1000 m above sea level is 239,80893 mg/gram sample and caffeine content in 1200 m above sea level is 185,93200 mg/gram. The highest caffeine contain is in height 800 m above sea level is 562,20782 mg/gram sample.
Co-Authors Astrid Octaviani Discus Hendra Setiawan Fea Prihapsara Nestri Handayani Nisya Arienda Rika Triyoga Prasetyo Rini Wahyu Rohmatin Nikmah