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T. Sembiring
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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BIOOEGRAOASI SENYAWA AROMATIK OLEH PSEUDOMONAS SP ISOLAT I4 DARI TABLET BAKTERI T. Sembiring; Lies Sriwuryandari
Jurnal Kimia Terapan Indonesia Vol 10, No 1-2 (2000)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4214.597 KB) | DOI: 10.14203/jkti.v10i1-2.182

Abstract

Biodegradation test of aromatic compounds by pure cultureof bacterial isolate I4 from bacterial tablet using mineral mediumas growth medium was done. Aromatic compounds tested ascarbon sources used were phenol, p-chlorphenol, p-nitrophenol,2,4 dichlorphenol and benzene. The oval colony forming and highmotility isolate is an aerobic and gram negatif bacteria. Theisolate was able to use citric acid as carbon source, producedacid .from methyl red test and gave negative result of indol test.The isolate formed a slightly blue-green colour colony on agarslant, and it did not use ethanol as carbon source. Thecharacteristic of the isolate I4 indicated that it belonged to thegenus Pseudomonas. Test using selective media confirmed thatthe isolate I4 is a Pseudomonas fluorescence. The capability ofthe bacteria to oxidize of 2,4 dichlorphenol was relatively fast.Experimental results showed that the bacteri grew on benzene atconcentration of 10% (v/v), and degraded 2,4 dichlophenol withrelatively high rate.Keywords: Bacterial tablet, aromatic compounds, phenol. pchlorphenol,2,4 dichlorphenol, p-nitrophenol, benzene, pure culture,Pseudomonas fluorescence, isolate I4
KARAKTERISASI PHARSIAL ISOLATBAKTERI PENDEGRADASI PHENOL DARI TABLET BAKTERI T. Sembiring; A. Susilorukmi
Jurnal Kimia Terapan Indonesia Vol 9, No 1-2 (1999)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3401.002 KB) | DOI: 10.14203/jkti.v9i1-2.199

Abstract

An aerobic pure culture (18) was isolated from the tablet of mixed culture bacteria which is originated from a farm land. The gram positive isolate able to degrade phenol about 550 mg/l.d. Morphology of the isolate changes with the culture ages. it was in rod shape during the logarithmic growth phase and changes to the o valform during the stationary phase. The range of temprature growth is 27-34°C and the optimum temprature of the isolate is 30°C with doubling time about 7 hours. Substrate could be utilized by the isolate are alcohol, aromatic compound and carbohydrate. Methanol, ethanol, isopropanol (each 10%; v/v), 4-Chlor phenol (0.2 mM), 4-nitrophenol(0.25 mM), anniline (1 mM)  and glucose are some substrate has been tested for the bacterial growing. Salt (NaCl) tolerance for the isolate is up to 2% (v/v) and the addition of ammonium salt would be support the bacterial growthKey words:Bacterial tablet, aerobic, phenol degradation, 4-chlor phenol, 4-nitro phenol, anniline, glucose, methanol, ethanol, iso-propanol, isolation, partial characterization.
Co-Authors A. Susilorukmi Lies Sriwuryandari