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All Journal Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam (JURRIMIPA) Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran
Rifdah Rifdah
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Dentistry Health Professions Mathematics Public Health

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Uji Efektivitas Daun Bungur (Lagerstroemia speciosa L.) terhadap Penghambatan Transportasi Glukosa Tikus Putih Galur Wistar (Rattus norvegicus) Haryanto Haryanto; Muhammad Halim Ramadhan; Rifdah Rifdah; Dian Indra Aulia; Harianti Harianti; Nadia Azzahra
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 3 No. 2 (2025): Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Pusat Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v3i2.4913

Abstract

This study investigated the effects of bungur leaves containing saponins, flavonoids and tannins on glucose and cholesterol levels in rat (Rattus norvegicus) test animals. Spectrophotometry results showed a significant decrease in glucose levels at different concentrations. SPSS analysis indicated that the data were normally distributed, and comparison of average glucose levels between treatment groups showed similar homogeneity. The significant value of bungur obtained was 0.014 < 0.05 from the ANOVA test, strengthening the conclusion that bungur leaves can contribute to glucose control which aims to increase the potential of active compounds contained in bungur samples in inhibiting blood glucose absorption.
Identifikasi Metabolit Sekunder Ekstrak Etanol Belimbing Wuluh (Averrhoa bilimbi. L) Fhadlyatul Khusna; Dhini Andriani; Fitrinaya Fitrinaya; Rifdah Rifdah; A. Ilmi Alfiana; Purnamasari Purnamasari; Friska Adelia Putri
JURNAL RISET RUMPUN MATEMATIKA DAN ILMU PENGETAHUAN ALAM Vol. 5 No. 2 (2026): Agustus : JURRIMIPA: Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrimipa.v5i2.9898

Abstract

Belimbing wuluh (Averrhoa bilimbi L.) is a medicinal plant known to contain various secondary metabolites, particularly flavonoids, which possess potential biological activities. This study aimed to isolate flavonoid compounds from the ethanol extract of belimbing wuluh fruit and determine the total flavonoid content. The research was conducted through sample preparation, ethanol extraction by maceration, solvent evaporation, liquid-liquid fractionation using n-hexane, ethyl acetate, and water, followed by phytochemical separation using column chromatography, preparative thin-layer chromatography, and two-dimensional thin-layer chromatography. The isolated fraction was further analyzed, and the total flavonoid content was determined using UV-Visible spectrophotometry with quercetin as the reference standard. The extraction process produced an extract yield of 22.70%, while solvent evaporation resulted in a concentrated extract with a yield of 8.8%. Fractionation indicated that the polar fraction was obtained in the highest proportion. Column chromatography generated 50 fractions with different color characteristics, indicating successful separation of compounds based on polarity. Thin-layer chromatography showed an Rf value of 0.4 in the ethyl acetate mobile phase, while preparative and two-dimensional thin-layer chromatography produced a single dominant band, suggesting a relatively pure isolate. The UV-Visible analysis showed excellent linearity and revealed a total flavonoid content of 230.76 mg QE/g or 23.08%, exceeding the minimum quality standard. These findings demonstrate that belimbing wuluh fruit is a promising natural source of flavonoids and has potential for further pharmaceutical and phytochemical development.
Co-Authors A. Ilmi Alfiana Dhini Andriani Dian Indra Aulia Fhadlyatul Khusna Fitrinaya Fitrinaya Friska Adelia Putri HARIANTI Harianti Haryanto Haryanto Muhammad Halim Ramadhan Nadia Azzahra Purnamasari Purnamasari