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J. Kantasubrata
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Studi Kromatografi Lapis Tipis Preparatif pada Pelat Silika dan Kromatografi Cairan Kinerja Tinggi pad a Kolom C18 dari Senyawa-Senyawa Hasil Biokonversi Solasodine J. Kantasubrata; T. Y. Fitri; V. A. Halomoan; Buchori Buchori; A. T.Karossi
Jurnal Kimia Terapan Indonesia Vol 4, No 2 (1994)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5483.7 KB) | DOI: 10.14203/jkti.v4i2.282

Abstract

The separation of solasodine bioconversion products-after fermentation with Mycobacterium phlei DSM 43286 has been carried out, using preparative thin layer chromatography on silica plate with chloroform-ethanol (48:1) mixture as an eluent. Chromatographic cross check of the compound being separated has also been done. In addition to the silica stationary phase, the separation of bioconversion products using CIS has also been explored. Solanesol and six derivatives of androstane or androstene standards could be well separated on Ci8 plate using methanol-chloroform (4:1). For the High Performance Liquid Chromatography (HPLC) separation on C18 column, the mixture of methanol-water was used instead of methanol-chloroform, since with the latter eluent, compounds being separated were eluted together with the solvent peak. The optimum resolution of solasodine bioconversion products could only be attained when the gradient elution technique using the mixture of methanolwater was used.
SOLASODINE STEROID BIOCONVERSION BY MYCOBACTERIUM PHLEI DSM 43286. S. Pudjiraharti; T. A. Budiwati; J. Kantasubrata; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2080.766 KB) | DOI: 10.14203/jkti.v2i1-2.280

Abstract

Bioconversion of solasodine by Mycobacterium phlei DSM 43286 was conducted to obtain intermediate compounds which might be used as precursor in the production of steroidal drugs, i:e androst-4-en-3, 17-dione (AD) and androsta-l,4-diene-3,17-dione (ADD). M. phlei was firstly grown in nutrient broth medium at 37 °C for 8.5 hours with agitation of 200 rpm. The bacterial culture thus obtained was used as starter to inoculate the conversion medium containing 0,02% solasodine as the substrate and 0.01% 8-hydroxyquinoline as inhibitor. Bioconversion was conducted for 12 days at 37 °C using the same speed of agitation. Analysis of the bioconversion products was carried oUl using samples taken periodically at a 24-hour interval by TLC and HPLC methods. TLC analysis using chloroform-ethyl acetate (80:20) as eluent, measurement of the nuvamum wavelength and molar extinction coefficient value showed that AD and ADD was not found in the fermentation product,, but other intermediau: compound might the present. However, HPLC analysis of the fermentation products using Ik'Porasil column and benzene- ethylacetatechloroform (40:80:10) as eluent, showed -peaks with retention time similar to that of AD (during the 2nd - 9th day of incubation) and, ADD (during the 5th - 6th day offermentation) and, other unknown peaks.
Co-Authors A. T. Karossi A. T.Karossi Buchori Buchori S. Pudjiraharti T. A. Budiwati T. Y. Fitri V. A. Halomoan