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All Journal Jurnal Kimia Terapan Indonesia Pendas : Jurnah Ilmiah Pendidikan Dasar
Rini Noviyanti
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology Other

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PEMURNIAN GLUKOAMILASE DARI HASIL FERMENTASI KAPANG RHIZOPUS ORYZAE Linar Z. Udin; Rini Noviyanti; A. Sidik; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 4, No 1 (1994)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2799.989 KB) | DOI: 10.14203/jkti.v4i1.250

Abstract

Purification of the glucoamylase R. oryzae was carried out by addition of ammonium sulfate 80% saturation, on the fermentation broth at 4°C. The precipitate formed by centrifugation at 9000 rpm was then dialyzed in buffer solution and then concentrated using freeze dryer. It was found that the specific activity of the enzyme was around three-fold higher the crudeenzyme from fermentation broth and the purity of the enzyme was almost twelve-fold.purer than the crude enzyme. The molecular weight of the glucoamylase was found to be 36,000 as determined by SDS gel electrophoresis. The optimum pH witli soluble starch as substrate was at pH 4.5 and the optimum temperature was 55°C while the Km Value was 0.027%.
UPAYA MENINGKATAN PEMAHAMAN KONSEP PADA MATERI GAYA DISEKITAR KITA MELALUI MODEL PEMBELAJARAN PROBLEM BASED LEARNING (PBL) PADA SISWA KELAS IV SDN SOROGOL Rini Noviyanti
Pendas : Jurnal Ilmiah Pendidikan Dasar Vol. 11 No. 03 (2026): Volume 11 No. 03, September 2026 Release
Publisher : Program Studi Pendidikan Guru Sekolah Dasar FKIP Universitas Pasundan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/jp.v11i03.58652

Abstract

The low level of students' conceptual understanding regarding "Forces Around Us" in the IPAS subject serves as the primary driver for this study. This issue stems from the dominance of conventional, teacher-centered approaches, which inadvertently restrict active student engagement. Addressing this challenge, this study aims to escalate the conceptual understanding of fourth-grade students at SDN Sorogol through the implementation of the Problem-Based Learning (PBL) model. A Classroom Action Research (CAR) utilizing the Kemmis & McTaggart cyclical design was applied as the research methodology, encompassing planning, action, observation, and reflection phases in each cycle. A total of 27 fourth-grade students at SDN Sorogol during the 2025/2026 academic year were involved as research subjects. Quantitative data regarding conceptual understanding were extracted via formative tests at the end of each cycle, while qualitative data concerning classroom activity dynamics were gathered through observation sheets. The empirical results confirmed a significant positive trend; in the pre-cycle stage, classical mastery only touched 18.5% (5 students) with a mean score of 60. Following the PBL model intervention in Cycle I, the mastery rate jumped to 59.2% (16 students) with a mean of 71. Optimal success was achieved in Cycle II, where classical mastery was accelerated to reach 88.8% (24 students) with a class mean score of 82. Based on these findings, it can be concluded that the integration of the Problem-Based Learning model effectively strengthens elementary school students' conceptual understanding of force materials.
Co-Authors A. Sidik A. T. Karossi Linar Z. Udin