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Muljadji Agma
2loniversitas Padjadjaran, Bandung, Jawa Barat, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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OPTIMASI DAN VALIDASI METODA PENGUJIAN WEDELOLAKTON SECARA KROMATOGRAFI CAIR KINERJA TINGGI DENGAN TEKNIK DETEKSI FLUORESENSI (KCKT - F) Iiisna Yuliana; Yosi Aristiawan; Iwan Bastiawanz; Julia J. Kantasubrata; Muljadji Agma
Jurnal Kimia Terapan Indonesia Vol 14, No 1 (2012)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4921.752 KB) | DOI: 10.14203/jkti.v14i1.114

Abstract

Wedelolakton has a wide range of biologicalactivities and used for the treatment of variousailments. In the present study an experiment ofquantitative determination of wedelolactone inherbal extract of Eclipta alba L. Hask has beendeveloped by using RP - HPLC - Fluorimetrytechnique and mixture methanol:0.35% acetic acid(H20) (50:50 vlv) as mobile phase, applied to theherbal extract as inhouse reference materialcandidate samples. The method was validated forthe confirmation of identity (selectivity), linearity,precision, recovery, sensitifity, limit of detectionJKTI, Vol. 14, No.1, Juni 2012and limit of quantitation. Statistical analysis of thedata showed that the method is reproducible andselective for the estimation of wedelolactonedetermination.Keywords: Wedelolactone, HPLC-F, method optimization,method validation, confidence limi
Co-Authors Iiisna Yuliana Iwan Bastiawanz Julia J. Kantasubrata Yosi Aristiawan