Putri Lili Epriyani
ahasiswa Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, IPB

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PENENTUAN KONDISI OPTIMUM FERMENTASI PADAT Trichoderma hamatum PADA MEDIA TUMBUH DEDAK PADI DALAM PRODUKSI SELULASE MENGGUNAKAN RESPONSE SURFACE METHODOLOGY Teuku Beuna Bardant; Haznan Abimanyu; Putri Lili Epriyani
Jurnal Kimia Terapan Indonesia Vol 15, No 2 (2013)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5932.591 KB) | DOI: 10.14203/jkti.v15i2.109

Abstract

Optimum condition for solidfermentation of Trichoderma hamatum inrice bran in order to produce cellulase hadbeen studied by using Response Surfacemethodology (RSM). Fermentationcondition variables that observed waswater to rice bran ratio, urea andinoculums additions cellulase activity inFPU/gds (gram of dry solid) became theonly dependent variable. The result ofempirical equation from this study wasproven to be valid within water to ricebran ratio 0,5 - 0,7, urea and inoculumsaddition where 2-3 % and 1-2% of ricebran weight respactively. Maximumcellulase activity that can be obtain withinthese ranges was 4.99 ± 0.92 FPU/gds.Keywords: Cellulase, Trichodermahamatum, rice bran, solid fermentation