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Kyoko Adachi
Marine Biotechnology Institute Co. Ltd., Kamaishi Lab.,3-75-1 Heita, Iwate 026-0001, Kamaishi City
Chemical Engineering, Chemistry & Bioengineering Chemistry

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THE ANTIBACTERIAL COMPOUND COLLISMICIN A DERIVED FROM MARINE Streptomyces sp Q-629K Tutik Murniasih; Kyoko Adachi
Indonesian Journal of Chemistry Vol 8, No 3 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1675.632 KB) | DOI: 10.22146/ijc.21600

Abstract

In our course of screening for secondary metabolite derived from marine bacterial, we isolate the antimicrobial compound collysmicin A from the ethyl acetate extract of Streptomyces sp Q-629K. Separation of this compound was carried out by silica gel open column chromatography. Purification of an active compound was done using HPLC C18 with acetonitril-water system. Determination of chemical structure was done by 1H, 13C NMR and LC-MS analysis. Collysmicin A was contained in fraction 3, fraction 7.2 and fraction 8.7. The antimicrobial assayed from purified compound Fr.8.7 gave diameter inhibition approximately 13 mm against S. aureus and 12 mm against B. subtilis.
Co-Authors Tutik Murniasih