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Klaus Günther
Institute for Chemistry and Dynamics of the Geosphere: ICG-III Phytosphere Research Centre Juelich, D-52425 Juelich
Chemical Engineering, Chemistry & Bioengineering Chemistry

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CAPILLARY ELECTROPHORETIC ANALYSIS OF LOW-MOLECULAR-MASS OF CA SPECIES IN PHLOEM SAP OF Ricinus communis L. Noor Fitri; Björn Thiele; Klaus Günther; Buchari Buchari
Indonesian Journal of Chemistry Vol 6, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.628 KB) | DOI: 10.22146/ijc.21757

Abstract

A capillary electrophoretic (CE) analysis with ultra-violet (UV) detection was performed for further separation of low-molecular-mass (LMM) calcium species in phloem sap of Ricinus communis L. Two different background electrolytes (BGE) were used for the separation; these are (1) hydrogen phosphate/dihydrogen phosphate buffer containing cetyltrimethylammonium bromide (CTAB) as an electro-osmotic flow (EOF) modifier, and (2) boric acid buffer containing CTAB. Various parameters affecting the analysis, including the composition and pH of the BGE were systematically studied. The sensitivity, resolution, baseline noise, migration time of the species peaks, and reproducibility of the method were evaluated under optimised condition. At least 13 UV-active species were optimally separated within about ten minutes. The optimised measurement condition was also achieved using 10 mM hydrogen phosphate/10 mM dihydrogen phosphate containing 0.5 mM CTAB at pH 8.0 as BGE, and by applying voltage of ‑20 kV and temperature of 14°C. Evidently, the analytical method was successfully used for the separation of LMM calcium species in phloem sap of R. communis L.
Co-Authors Björn Thiele Buchari Buchari Noor Fitri