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All Journal International Journal of Biosciences and Biotechnology Jurnal Ilmu Dasar Jurnal Kimia Riset
Siswoyo Siswoyo
Department of Chemistry, Faculty of Mathematics and Sciences, University of Jember Jl. Kalimantan 37 Kampus Tegalboto, Jember 68121
Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Environmental Science Mathematics

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SUGAR PRODUCTION BY DIGESTING OF OIL PALM EMPTY FRUIT BUNCH USING EXTRACELLULAR ENZYMES FROM Aspergillus niger AND Trichoderma reesei FOR ETHANOL PRODUCTION Kahar Muzakhar; Sutoyo S; Siswoyo S
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.426 KB)

Abstract

Extracellular enzymes which obtained from 4 days cultivation Aspergillus niger and Trichoderma reesei onsolid state fermentation of oil palm empty fruit bunch (OPEFB) were used for lignocellulosic-­??rich OPEFBdigestion. The enzymes were concentrated using 70% saturated ammonium sulphate, dialysed against20mM acetate buffer at pH 5 and adjusted one tenth (v/v) from the initial volume with the same buffer.The concentrated enzymes were then used in hydrolysation of powdered OPEFB. Amount of 10.65 mg/ mland 11.47 mg/ml sugars were produced when each concetrated enzyme A. niger and T. reesei mixedwith2%OPEFB. These hydrolysation were done on 100 ml total volume, incubated at 37oC with 100 rpmshaken for 36 hours. Further, both hydrolyzates results were sterilised and fermented anaerobically usingSaccharomycess cerevisiae at concentration 0.5mg/ml cells and incubated in 30oC for 24 hours. Colorimetricanalysis using QuantiChrom Kit DIET-­??500 at OD 580nm gave results the alcohol production were 0.86%and 0.92% which were similar with Gas Chromatograph analysis that of 0.83% and 0.93%, respectively.
OPTIMASI PARAMETER TEKNIK SQUARE WAVE VOLTAMMETRY DENGAN ELEKTRODA GRAFIT FAN PENERAPANNYA PADA ANALISIS KLORIN BEBAS DALAM AIR CUCIAN BERAS BERPEMUTIH Fenly Arismaya; Siswoyo Siswoyo; Asnawati Asnawati
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.588 KB) | DOI: 10.20473/jkr.v3i2.8704

Abstract

AbstrakKlorin (OCl-) merupakan bahan kimia berbahaya bagi kesehatan manusia, yang kadang digunakan sebagai bahanpemutih beras.  Oleh  sebab  itu  dilakukan  upaya  analisis  Klorin  menggunakan  teknik  Square  Wave  Voltammetry.  Penelitian  ini melakukan optimasi pengaturan parameter (konsentrasi larutan elektrolit, pH, dan sampling time) dan karakterisasi metode kerja dari teknik Square Wave Voltammetry, sehingga teknik ini dapat diaplikasikan untuk analisis klorin bebas (OCl-) dalam air cucian beras berpemutih. Variasi konsentrasi larutan elektrolit yang diterapkan dalam penelitian ini adalah 0,05; 0,10; 0,15; 0,20; 0,25 M. Variasi pH yang diterapkan pada pH 7, 8, 9, 10, 11, dan 12. Variasi sampling time yang diterapkan adalah 5, 10, 15, dan 20 milisekon. Berdasarkan hasil penelitian yang diperoleh, konsentrasi larutan elektrolit optimum pada konsentrasi 0,15 M, pH optimum pada kondisi pH 9, dan sampling time optimum adalah 10 milisekon. Hasil karakterisasi kinerja metode pada teknik Square Wave Voltammetry yang telah dioptimasi ini menghasilkan linieritas 0,9885, limit deteksi sebesar 9,563 ppm, dan nilai koefisien variasi sebesar 4,09%. Hasil pengukuran konsentrasi klorin bebas (OCl-) dengan teknik Square Wave Voltammetry yang telah dioptimasi adalah senilai 30,125 ppm. Pengaturan tiga parameter tersebut berpengaruh terhadap bentuk dan ketinggian puncak voltamogram yang dihasilkan. Kata Kunci: Square Wave Voltammetry, Optimasi Parameter, Klorin bebas, Beras Berpemutih.
Voltammetric Synthesis of Conducting Polymer Polypyrrole and Its Response Characteristic to Alcohol Susi Nurqomariyah; Asnawati Asnawati; Neran Neran; Siswoyo Siswoyo
Jurnal ILMU DASAR Vol 16 No 2 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.328 KB) | DOI: 10.19184/jid.v16i2.1485

Abstract

Conducting polymer is a used material for many purposes, including active compound of chemical sensor. Polypyrrole, one type of conducting polymers, is frequently used because of its advantages, namely owing high conductivity, strong mechanical properties and relatively stable compound.  This research was aimed to develop an alcohol sensor based on polypyrrole. Electropolymerisation of the polypyrrole was carried out using cyclic voltammetric technique. This research investigated some parameters electropolymerisation, namely variation of dopant electrolyte concentration, potential scan-rate, surface morphology of the polymer resulted, characteristic of the sensor performance when exposed to some alcohol compounds. According to the result of investigation, it was found that variation of potential scan-rate and dopant concentration has significant effect to the electropolymerisation process as well as to the resulted polymer, as indicated by the voltammogram profiles, the surface morphology of the resulted polymer and the response of resistance change of the polymer when exposed to the alcohol compounds.Keywords: polypyrrole, conducting polymer, alcohol sensor, cyclic voltammetry, electropolymerisation.
FLOW INJECTION POTENTIOMETRIC ANALYSIS OF ASPARTIC ACID, GLUTAMIC ACID AND ASCORBIC ACID USING PLATINUM ELECTRODES Yeni Maulidah Muflihah; Siswoyo Siswoyo; Tanti Haryati; Kiki Puji Setianingrum
Jurnal ILMU DASAR Vol 13 No 1 (2012)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.474 KB) | DOI: 10.19184/jid.v13i1.886

Abstract

The presence of  weak acids in solution can be detected using a potentiometric detector. Platinum was used as a working electrode and Ag/AgCl as a reference electrode. Ascorbic, glutamic and aspartic acid were detected by a platinum electrode in a flow potentiometric system. The influence of pH, flow rate and concentration of phosphate buffers asa a carrier were studied and showed an optimum pH for the detection of ascorbic and glutamic acid at pH 6,5 and pH 7,0 for aspartic acid. Phosphate buffer concentration optimum at 1x10-4M and flow rate of 1,00 mL/min. Linear range for ascorbic and glutamic acid at 2,5 x10-4M to 5x10-2M, with a regression coefficient of  0,974 and 0,958, while for aspartic acid 5x10-4M to 5x10-2M with a regression coefficient 0,911. Detection limit for ascorbic and glutamic acids were 5x10-4M and 1x10-3M for aspartic acid. Sensor reproducibility obtained from variation coefficient  (Kv). Variation coeffiecient (Kv) of ascorbic acids 1,32-1,69%, glutamic acids 0,69- 1,57% and aspartic acid 0,54- 1,29%.
Co-Authors Asnawati Fenly Arismaya Kahar Muzakhar Kiki Puji Setianingrum Neran Neran Susi Nurqomariyah Sutoyo S Tanti Haryati Yeni Maulidah Muflihah