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Christian W Huck
Institute of Analytical Chemistry and Radiochemistry, University of Innsbruck,
Chemical Engineering, Chemistry & Bioengineering Chemistry

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MONOLITHIC DISK FOR THE FAST CHROMATOGRAPHIC SEPARATION Nurul Hidayat Aprilita; Rania Bakry; Christian W Huck; Guenther K Bonn
Indonesian Journal of Chemistry Vol 5, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.06 KB) | DOI: 10.22146/ijc.21815

Abstract

Poly(styrene/divinylbenzene) (PS/DVB) monolithic disk was prepared by in situ free-radical copolymerization of styrene and divinylbenzene in the presence of decanol and tetrahydrofuran as porogens. PS/DVB monolithic disks were produced in two different lengths 1.5 mm and 3 mm. The disks were used in reversed phase chromatography of proteins with 0.2 % trifuoroacetic acid (TFA) and 0.2 % TFA in acetonitrile as mobile phase A and B, respectively. The effect of gradient rate, flow rate, temperature and disk length on the separation of proteins were also studied. PS/DVB monolithic disks allow the rapid separation of proteins in reversed phase chromatography.
Co-Authors Guenther K Bonn Nurul Hidayat Aprilita Rania Bakry