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All Journal Indonesian Journal of Chemistry
Hartiwi Diastuti
Department of Chemistry, Universitas Jenderal Soedirman, Jl. dr. Soeparno 61, Karangwangkal, Purwokerto 53123, Indonesia
Chemical Engineering, Chemistry & Bioengineering Chemistry

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Antibacterial Activity of Benzyl Benzoate and Crotepoxide from Kaempferia rotunda L. Rhizome Hartiwi Diastuti; Mochammad Chasani; Suwandri Suwandri
Indonesian Journal of Chemistry Vol 20, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.682 KB) | DOI: 10.22146/ijc.37526

Abstract

Benzyl benzoate and crotepoxide are the major components of Kaempferia rotunda L. rhizome. However, the bioactivity study of benzyl benzoate and crotepoxide as the antibacterial activity were still limited. Therefore, the antibacterial activity of benzyl benzoate and crotepoxide against four pathogenic bacteria, i.e., Escherichia coli ATCC 25922, Enterococcus aerogenes ATCC 13048, Bacillus cereus ATCC 6538 and Staphylococcus aureus ATCC 11778 were investigated. The isolation steps were include the extraction by maceration with acetone, then the acetone extract was partitioned with n-hexane:methanol (1:1) and ethyl acetate:water (1:1) respectively. The isolation by liquid vacuum chromatography followed by column chromatography was obtained benzyl benzoate from the n-hexane fraction and crotepoxide from ethyl acetate fraction. The molecular structure of isolated compounds was identified based on NMR (1D and 2D) spectroscopic data. The antibacterial activity assay of isolated compounds was carried out using the disc diffusion method. The antibacterial evaluation confirms that the benzyl benzoate and crotepoxide exhibits a medium level activity. Benzyl benzoate showed highest antibacterial activity against B. cereus at the MIC of 50 μg/mL and inhibitory zone of 5.9 mm, while the crotepoxide showed highest antibacterial activity against E. aerogenes at the MIC of 100 μg/mL with inhibitory zone 6.1 mm.
Design of Defect and Metallic Silver in Silver Phosphate Photocatalyst Using the Hydroxyapatite and Glucose Uyi Sulaeman; Suhendar Suhendar; Hartiwi Diastuti; Roy Andreas; Shu Yin
Indonesian Journal of Chemistry Vol 20, No 6 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.48647

Abstract

The defect and metallic silver (Ag) in silver phosphate (Ag3PO4) photocatalyst were successfully generated using hydroxyapatite (HA) and glucose. Two steps of synthesis were done in these experiments. Firstly, the Ag/HA powder was prepared by reacting AgNO3 and HA, followed by the addition of a glucose solution. Secondly, the suspension of Ag/HA was reacted with AgNO3 aqueous solution. The yellow product of Ag/Ag3PO4 photocatalyst was produced. The products were characterized using X-Ray Diffraction (XRD), Diffuse Reflectance Spectroscopy (DRS), Scanning Electron Microscope (SEM), Brunauer–Emmett–Teller (BET) and X-ray Photoelectron Spectroscopy (XPS). The decreased ratio of O/Ag and metallic Ag formation observed by the XPS was detected as the possible defect and Ag-doping in the photocatalyst. The enhanced photocatalytic activity might be caused by the oxygen vacancy and metallic Ag in Ag3PO4 that enables the separation of photo-generated electrons and holes.
Co-Authors Mochammad Chasani Roy Andreas Shu Yin Suhendar Suhendar Suwandri Suwandri Uyi Sulaeman