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Farhan Mohd Said
Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Pahang, Malaysia
Chemical Engineering, Chemistry & Bioengineering Chemistry

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Effect of Key Parameters on Jacalin Extraction from Aqueous Phase into Anionic Reverse Micelles Sharifah Fathiyah Sy Mohamad; Farhan Mohd Said; Mimi Sakinah Abdul Munaim; Shahril Mohamad
Indonesian Journal of Chemistry Vol 19, No 3 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.855 KB) | DOI: 10.22146/ijc.39705

Abstract

This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture.
Co-Authors Mimi Sakinah Abdul Munaim Shahril Mohamad Sharifah Fathiyah Sy Mohamad