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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Chemistry
Fahrul Zaman Huyop
Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malaysia
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science

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Further Analysis of Burkholderia pseudomallei MF2 and Identification of Putative Dehalogenase Gene by PCR Mohamed Faraj Edbeib; Roswanira Abdul Wahab; Fahrul Zaman Huyop; Hasan Murat Aksoy; Yilmaz Kaya
Indonesian Journal of Chemistry Vol 20, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.629 KB) | DOI: 10.22146/ijc.43262

Abstract

Halogenated organic compounds are extensively and widely used as pesticides, herbicides, and antibiotics that contribute to the pollution. This research was aimed to further analyze and characterize a bacterium that has the ability to utilize 2,2-dichloropropionic acid (2,2-DCP) as a model to study dehalogenase enzyme production.  Microscopic observation, biochemical tests and PCR technique were carried out in order to characterize the isolated bacterium. Strain MF2 showed its ability to grow on 10 mM 2,2-DCP liquid minimal medium with doubling time of 13 h with maximum chloride ion released of 19.8 molCl–/mL. The 16S rDNA analysis suggested that strain MF2 belongs to the genus Burkholderia. This was supported by the microscopic observation and biochemical tests. Dehalogenase gene was observed when using only primers dehIfor1 and dehIrev2 derived from group I deh PCR primer sequences, whereas no amplification using dhlB-314-forward and dhlB-637-reverse (group II dehalogenase) and haloacetate dehalogenase (H2-1157-forward and H2-1662-reverse) PCR primer sequences. The results suggested that, possibly, dehalogenase from MF2 was related to group I deh. In conclusion, strain MF2 showed the ability to utilize 2,2-DCP as sole source of carbon and energy. Further analysis revealed the MF2 strain consisted of dehalogenase gene that could be used for degradation of man-made halogenated compounds present in the environment. Using existing dehalogenase PCR primers, it was possible to amplify the dehalogenase genes sequence.
Post-Covid-19 Pandemic Awareness on The Use of Micro- and Nano Plastic and Efforts into Their Degradation - A Mini Review Ekwan Nofa Wiratno; Amira Azawani Mohd Rozdhl; Nafizatun Eliana Ali Hanafi; Rabiatul Alia Redzuan; Fahrul Zaman Huyop
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.12

Abstract

Micro- and nanoplastic pollution possess a global threat and cause a future problem and needs greater attention. Its pollution is also exacerbated recently by the use of abundance of plastic polymers in efforts to prevent and handle COVID-19 pandemic at the global scale. This review covered the major concerns about the characteristic, effect and bioremediation of micro- and nanoplastics of post COVID-19. Based on size, microplastic is described as debris particles smaller than 5 mm whereas, nanoplastic is referred to any particles smaller than 100 nm. Micro- and nanoplastic are easily ingested by many aquatic organisms at different trophic levels. This ingestion caused negative health impacts to all living organisms. Microplastic direct effect on living organism for example mechanical injury, false satiation, declined growth, promoted immune response, energy loss, disrupted enzyme activity and production, decreased fecundity, production of oxidative stress, and mortality. Nanoplastic could enter the circulatory system and caused negative effects on the cellular and molecular levels. Bioremediation of microplastic by magnoliophyta, bacteria, fungus and algae on several polymer forms was previously reported, however, not many on nanoplastic biodegradation. Therefore, current review will focus on the characteristics, effect and bioremediation effort of micro- and nanoplastic.
Co-Authors Amira Azawani Mohd Rozdhl Ekwan Nofa Wiratno Hasan Murat Aksoy Mohamed Faraj Edbeib Nafizatun Eliana Ali Hanafi Rabiatul Alia Redzuan Roswanira Abdul Wahab Yilmaz Kaya