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Mary Astuti
Gadjah Mada University
Agriculture, Biological Sciences & Forestry

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Hemoglobin Regeneration and Distribution of Iron, Copper and Zinc in Hepatic Subcelluler Frantions of Rats Fed With Tempe Diets Mary Astuti; Seiichi Kasaoka; Shiro Goto; Mochamad Adnan
Indonesian Food and Nutrition Progress Vol 1, No 1 (1994)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.6

Abstract

Hemoglobin regeneration efficiency was used to predict the iron availability of tempe diet by the method of depletion and repletion using rats for animal experiment. Hemoglobin regeneration efficiency of tempe showed the same effect with casein diet and unfermented soybean diet indicating that iron from soybeans are a good source of available iron. Iron, copper, and zinc from casein, unfermented soybean and tempe diets were distributed in hepatic subcelluler fractions in the same pattern. The highest level of iron and zinc were found in the cytosolic fraction and copper was found in the nuclear fraction. The different pattern among those trace minerals indicates that they have distinct roles in hemoglobin synthesis and metabolism.
Quenching Mechanisms and Kinetics of Quercetin in Inhibition of Photosensitized Oxidation of Palm Oil and Linoleic Acid Posman Sibuea; Suparmo Suparmo; Umar Santoso; Zuheid Noor; Mary Astuti; Sri Raharjo
Indonesian Food and Nutrition Progress Vol 11, No 2 (2004)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.39

Abstract

Effect of 0, 200, 400, 600, 800 and 1000 ppm (wt/vol) quercetin on the erythrosine sensitized photooxidations of palm oil and linoleic acid in methylene chloride containing 100 ppm erythrosine, were studied during storage under 4000 lux fluorescent light for 5 h by measuring peroxide value. Steady-state kinetic approximation was used to determine a quenching mechanism and quenching rate constant of quercetin in the erythrosine-sensitized photooxidation of palm oil and linoleic acid in methylene chloride model system. Erythrosine greatly increased the photooxidation of palm oil and linoleic acid, as was expected. Quercetin was extremely effective in minimizing erythrosine-sensitized photooxidation of palm oil and linoleic. As the concentration of quercetin increased from 0 to 200, 400, 600, 800 and 1000 ppm, the peroxide values of palm oil and linoleic acid decreased significantly (P <0.05). The steady-state kinetic studies indicated that quercetin quenched singlet oxygen only to minimize tire erythrosine-sensitized photooxidation of palm oil and linoleic acid. The calculated total quenching rate of quercetin on erythrosine photosensitized oxidation of palm oil in methtylene chloride was 4.3 x 109 M-1s-1 and total quenching rate of quercetin on erythrosine photosensitized oxidation of linoleic acid in methtylene chloride was 3.2 x 109 M-1s-1.
Co-Authors Mochamad Adnan Posman Sibuea Seiichi Kasaoka Shiro Goto Sri Raharjo Suparmo Suparmo Umar Santoso Zuheid Noor