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R Wasito
Gadjah Mada University
Agriculture, Biological Sciences & Forestry

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The Promoting Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-Dioxin on Nasal Tumors in Sprague-Dawley Rats R Wasito
Indonesian Food and Nutrition Progress Vol 1, No 2 (1994)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2086.495 KB) | DOI: 10.22146/jifnp.20

Abstract

It was hypothesized that exposure to environmentally relevant dietary levels of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) or 2, 2', 4, 4', 5, 5'-hexachlorobiphenyl (245- HCB) could cause the development of nasal tumors and a combined exposure to these compounds could have a potentiating effect on tumor development. Thirty days after partial hepatectomy and NDEA administration, groups of 12 or 24 female Sprague-Dawley rats were fed a basal diet or diets containing 10 ppt TCDD, 10 ppt TCDD, 5 ppm 245-HCB, 10 ppt TCDD and 245-HCB or 100 ppt TCDD and 245-HCB for 140 days. Basal diets were fed from day 140 unit rats were killed on day 210 or day 420. Results indicated that TCDD or a combination of TCDD and 245-HCB significantly increased the incidence of nasal tumors by day 420. However, a combined exposure to TCDD and 245-HCB had no potentiating effect on the incidence of nasal tumors.
Diagnostic Value of Lipoprotein (a) in Cardiovascular Yanuartono Yanuartono; Hastari Wuryastuti; R Wasito
Indonesian Food and Nutrition Progress Vol 5, No 2 (1998)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.64

Abstract

This research was conducted to evaluate cardiovascular disease caused by atherogenic diets, based on immunological assay by measuring the concentration of lipoprotein (a)/Lp (a). Eighty male Sprague Dawley rats, 150-200 grams' of body weight and three months of age were used in this research. The rats were randomly alloted into four groups, 20 of each. Group I as control was fed normal diet, group 11 was fed diet containing high cholesterol, group 111 was fed diet containing high fat and group 117 was fed diet containing high cholesterol and high fat (atherogenic). After 2, 4, 8, 16 weeks on experimental diet, 20 rats were selected randomly (5 rats of each group), and blood sample were withdrawn for Lp (a) analysis. All animal were then killed and the heart were taken out for histopathological analysis. The statistical analysis for Lp (a), data showed that there were significant differences (p<0.05) among all of the treatments, high fat diet had the greatest influence on Lp (a) concentration. It can be concluded that Lp (aconcentration could be influence by high fat diet, but not by period of treatment. Lp (a) concentration seems connected with the incidence of atherosclerosis in rats. For this reason, evaluation of Lp (a) concentration could be considered as a routine procedure in general health evaluation.
The Role of Intrinsic Pathway Apoptosis via Caspase-9 in Atherogenesis Due To Atherogenic Diet in Sprague Dawley Rats Yanuartono Yanuartono; Hastari Wuryastuti; R Wasito; Sri Raharjo
Indonesian Food and Nutrition Progress Vol 12, No 1 (2005)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.32

Abstract

Thirty male rats, strain Sprague Dawley were used as experimental animal to study the role of intrinsic (mitochondrial) pathway apoptosis in atherogenesis due to high fat and high cholesterol diet. The rats were randomly alloted into three group (I, II, III) of 15 each. Group I as control was fed normal diet, group II was fed diet containing high fat diet, and group III was fed containing high fat and high cholesterol diet (atherogenic diet). After 6 and 12 weeks on experimental diet, 15 rats were selected randomly (5 rats of each group). All animal were then killed and the aorta were taken out for caspase-9 immunohistochemical analysis. Based on the present study result it can be concluded that high fat diet and high cholesterol diet could induced apoptosis through caspase-9.
The Role of Extrinsic Pathway (Death Receptor Pathway) Apoptosis through Caspase-8 in Atherogenesis due to High Fat and High Cholesterol Diet Yanuartono Yanuartono; Hastari Wuryastuti; R Wasito; Sri Raharjo
Indonesian Food and Nutrition Progress Vol 12, No 1 (2005)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.33

Abstract

Thirty male rats, strain Sprague Dawley were used as experimental animal to study the role of death receptor pathway apoptosis in atherogenesis due to high fat and high cholesterol diet. The rats were randomly alloted into three group (I, II, III) of 10 each. Group I as control was fed normal diet, group II was fed diet containing high fat diet, and group III was fed containing high fat and high cholesterol diet (atherogenic diet). After 6 and 12 weeks on experimental diet, 15 rats were selected randomly (5 rats of each group). All animal were then killed and the aorta were taken out for caspase-8 immunohistochemical analysis. Based on the present study result it can be concluded that high cholesterol and/or high fat diet induced apoptosis through death receptor pathway via caspase-8.
Co-Authors Hastari Wuryastuti Sri Raharjo Yanuartono, Yanuartono