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D.L Pyle
University of Reading
Agriculture, Biological Sciences & Forestry

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Kinetics and Substrate Partitioning in the Polyphenol Oxidase-Catalysed Oxidation of Catechol in a Two-Phase System Tyas Utami; D.L Pyle
Indonesian Food and Nutrition Progress Vol 5, No 1 (1998)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.263 KB) | DOI: 10.22146/jifnp.56

Abstract

The kinetics of catechol oxidation catalysed by polyphenol oxidase in two-phase systems with mixtures of lauryl alcohol and hexane as the solvent phase is investigated, with particular reference to the effect of partitioning of catechol on the enzyme kinetics. Theory is developed to derive a relationship between the apparent K. and the intrinsic or 'true' Kn. The theory predicts that substrate partitioning should not change %Cue but that the relationship between the apparent and intrinsic K,, values should depend on the phase volume ratio and the partition coefficient. The theory shows good agreement with the results and gives a consistent K. value.
Co-Authors Tyas Utami