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All Journal ANNALES BOGORIENSES
Tri Muji Ermayanti
Research Centre for Biotechnology, Indonesian Institute of Sciences (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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The Effect of Increase in NaCl Concentration on Growth and Proline Content of Purple Yam (Dioscorea alata L.) Grown In Vitro Andri Fadillah Martin; Farroh Azizah; Dyah Retno Wulandari; Tri Muji Ermayanti
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3687.679 KB) | DOI: 10.14203/ann.bogor.2012.v16.n2.13-18

Abstract

Tuber of purple yam (Dioscorea alata L.) has been used as an alternative food in some areas in Indonesia. The tuber contains high carbohydrate, has low glycemic index and is gluten free. Therefore, study on genetic improvement of this species needs to be carried out in order to increase its productivity and to find out new cultivars which can be cultivated in marginal lands. This research was aimed to investigate the effect of NaCl concentration on growth and proline content of purple yam grown in vitro. Shoot tips were cultured on MS (Murashige and Skoog) medium supplemented with NaCl at concentrations of 25; 50; 100; 200 and 250 mM. After six weeks in culture, height of shoots, number of nodes, number of leaves, as well as proline content were recorded. The results showed that shoots grown on MS medium supplemented with NaCl at 25 and 50 mM had better growth compared to control. The best medium for its growth was MS containing 50 mM of NaCl. An increase in NaCl level resulted in decrease of growth. The LD50 value was obtained at 183 mM of NaCl. The highest proline concentration was achieved by shoots grown on the medium supplemented with 100 mM of NaCl. This result indicated that purple yam was tolerant to the increase of NaCl concentration up to 100 mM on MS medium without addition of plant growth regulators. Keywords: purple yam, Discorea alata L., NaCl, in vitro growth, proline content.
Cytological Analysis of Root Cultures of Artemisia Cina Tri Muji Ermayanti; Oktavia Yanti; Erwin Al Hafiizh
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4020.397 KB) | DOI: 10.14203/ann.bogor.2004.v9.n2.50-58

Abstract

Artemisia cina is a medicinal plant species producing bioactive compounds which are potential as antitumor, antifungal and antibacterial. The aim of this study was to analyze the stabililY of chromosome number in root cultures of A. cina. Transformed root culture was established by infection of leaves of A. cina with Agrobacterium rhizogenes strains 07-20001 . ATCC-15834. A4 and A. tumefaciens strain RlOOO . Roots isolated from glasshouse plants, plantlets grown in solid and liquid MS medium were utilized for investigation of chromosome examination of untransformed roots. Chromosome examination was conducted by squashing method and chromosome numbers were calculated under microscope. The .results showed that both untransformed and transforme root had instability in the chromosome number, but had the modal number of chromosome x=8 with the diploid number of 2n = 4x = 32. Roots isolated from glasshouse plants of A. Cina had 53.7% of cell with the diploid numbers of 2n = 32 and 46.3% of cells had chromosome numbers ranged from 2n = 12 to 2n = 64. Untransformed roots isolated from plantlets cultured in solid medw had only 36.1 % or cells with chromo orne number of 2n = 32, and unlran fomled ro t5 grown in liquid medium had 49.4% of cells with 2n = 32. The chromosome numbers of A. Cina transformed roots was affected by trains of Agrobacterium. Root transformed with the bacterium Strain 07 -20001 showed lhe highest in normal chromosome number of 2n = 32 (62.4%) followed by roots lransformed wiLll strains ATCC-15834 (61.9 %). R1000 (43.6%) and A4 (43.0%). The range of the chromosome number untransformed roots was from 2n= 17 to 2n=64 whilst that of transformed roots was from 2n= 1 l to 20=66
Co-Authors Andri Fadillah Martin Dyah Retno Wulandari Erwin Al Hafiizh Farroh Azizah Oktavia Yanti