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Latifah K Darusman
Bogor Agricultural University, Kampus IPB Darmaga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Cellulolytic Yeast Isolated From Raja Ampat Indonesia Atit Kanti; Nampiah Sukarno; Endang Sukara; Latifah K Darusman
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.042 KB) | DOI: 10.14203/ann.bogor.2012.v16.n1.27-34

Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of  Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS) with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates  Sporobolomyces poonsookiae Y08RA07,  Rhodosporidium paludigenum Y08RA29 and Cryptococcus flavescens Y08RA33were 1.40, 2.60 and 1.66 respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07, whereas for Y08RA29 andY08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf.   Key words: Cellulolytic yeast, Raja Ampat, waste paper, bamboo leaf
Co-Authors Atit Kanti Endang Sukara Nampiah Sukarno