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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Floribunda Jurnal Ilmiah Sains
Nampiah Sukarno
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Physics Veterinary

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Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit; Sukarno, Nampiah; Sukara, Endang; Darusman, Latifah K
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.042 KB) | DOI: 10.1234/46

Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of  Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS) with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates  Sporobolomyces poonsookiae Y08RA07,  Rhodosporidium paludigenum Y08RA29 and Cryptococcus flavescens Y08RA33were 1.40, 2.60 and 1.66 respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07, whereas for Y08RA29 andY08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf.   Key words: Cellulolytic yeast, Raja Ampat, waste paper, bamboo leaf
Identification of Ectomycorrhiza-Associated Fungi and Their Ability in Phosphate Solubilization Mujahidah, Shofia; Sukarno, Nampiah; Kanti, Atit; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3741

Abstract

ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii 
IDENTIFICATION OF ECTOMYCORRHIZA-ASSOCIATED FUNGI AND THEIR ABILITY IN PHOSPHATE SOLUBILIZATION Mujahidah, Shofia; Sukarno, Nampiah; Kanti, Atit; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3741

Abstract

ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii 
Growth and Development of Tristaniopsis merguensis Seedling Inoculated by Natural Ectomycorrhiza Triadiati, Triadiati; Hidayanti, Anastastia Raditya; Sukarno, Nampiah
Journal of Tropical Life Science Vol 10, No 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.185 KB) | DOI: 10.11594/jtls.10.02.01

Abstract

Tristaniopsis merguensis (pelawan tree) is a potential plant, however, it has not cultivated and conserved optimally due to slow growth and lack of information about the cultivation. The existence of T. merguensis in the forest is important because pelawan fungi grow under the tree, particularly in the above of the roots. Information about how to grow the T. merguensis seedling is very limited; therefore, research in growth and development of T. merguensis seedling was important. The aims of this study were to determine the appropriate planting medium for T. merguensis seed germination and to stimulate growth and development of T. merguensis seedling by using its fungi. Status of T. merguensis fungi was studied by analyzing root morphology. Fungi isolation was carried out from colonized root and fungi fruit body. The stimulated the growth of T. merguensis seedling was done by using fungi isolated from colonization root in laboratory scale, as well as treated at a different phosphate concentration. The result showed that T. merguensis was associated with ectomycorrhizal fungi. Planting medium consists of sawdust and T. merguensis fine root resulted the highest percentage of germination. Seedlings were treated with ectomycorrhizal fungi grew better than without ectomycorrhizal fungi. In addition, seedlings treatment with ectomycorrhizal fungi and phosphate 25% showed the highest growth rate. 
Diversity of Endophytic Fungi from Red Ginger (Zingiber officinale Rosc.) Plant and Their Inhibitory Effect to Fusarium oxysporum Plant Pathogenic Fungi ROHANI CINTA BADIA GINTING; NAMPIAH SUKARNO; UTUT WIDYASTUTI; LATIFAH KOSIM DARUSMAN; SIHEGIKO KANAYA
HAYATI Journal of Biosciences Vol. 20 No. 3 (2013): September 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.046 KB) | DOI: 10.4308/hjb.20.3.127

Abstract

Indonesia has been known as a country with high medicinal plant diversity. One of the most common medicinal plant from Indonesia is red ginger (Zingiber officinale Rosc.). Nevertheless, limited studies of endophytic fungi associated with these medicinal plants are hitherto available. The objectives of this research were to study the diversity of endophytic fungi on red ginger and to analyze their potential as a source of antifungal agent. All parts of plant organs such as leaf, rhizome, root, and stem were subjected for isolation. Fungal identification was carried out by using a combination of morphological characteristic and molecular analysis of DNA sequence generated from ITS rDNA region. Thirty endophytic fungi were successfully isolated from leaf, rhizome, root, and stem of red ginger plant. Antagonistic activity was tested against Fusarium oxysporum, a pathogenic fungus on plants, using an antagonistic assay. Based on this approach, the fungi were assigned as Acremonium macroclavatum, Beltraniella sp., Cochliobolus geniculatus and its anamorphic stage Curvularia affinis, Fusarium solani, Glomerella cingulata, and its anamorphic stage Colletotrichum gloeosporoides, Lecanicillium kalimantanense, Myrothecium verrucaria, Neonectria punicea, Periconia macrospinosa, Rhizopycnis vagum, and Talaromyces assiutensis. R. vagum was found specifically on root whereas C. affinis, L. kalimantanense, and M. verrucaria were found on stem of red ginger plant.  A. macroclavatum was found specifically in red ginger plant’s organ which located under the ground, whereas C. affinis was found from shoot or organ which located above the ground. The antagonistic activity of isolated endophytic fungi against F. oxysporum varied with the inhibition value range from 1.4 to 68.8%. C. affinis (JMbt7), F. solani (JMd14), and G. cingulata (JMr2) had significantly high antagonistic activity with the value above 65%; and R. vagum (JMa4) and C. geniculatus (JMbt9) had significantly low antagonistic activity with the range value 0-10%.
Endophytic Fungi Associated With Turmeric (Curcuma longa L.) Can Inhibit Histamine-Forming Bacteria in Fish Eris Septiana; Nampiah Sukarno; . Sukarno; Partomuan Simanjuntak
HAYATI Journal of Biosciences Vol. 24 No. 1 (2017): January 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.129 KB) | DOI: 10.4308/hjb.24.1.46

Abstract

Turmeric (Curcuma longa L.) is a medicinal plant that is commonly used as spice and preservative. Many types of endophytic fungi have been reported as being associated with medicinal plants and able to synthesize secondary metabolites. In this study, endophytic fungi were isolated from all plant parts of turmeric plants. Identification of the endophytic fungi was done using morphological characteristics and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA. The dual culture method was used for screening antibacterial activity of the endophytic fungi against Morganella morganii, a common histamine-producing bacteria. The disc diffusion method was used to test the ability of water fractions of selected endophytic fungi to inhibit M. morganii growth. Two-dimensional thin layer chromatography was used to determine the fungal extract inhibition activity on histamine formation. In total, 11 endophytic fungi were successfully isolated and identified as Arthrobotrys foliicola, Cochliobolus kusanoi, Daldinia eschscholzii, Fusariumoxysporum, Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Phanerochaete chrysosporium, and Phaeosphaeria ammophilae. Five isolates showed inhibition activity against M. morganii in the dual culture tests. Based on the disc diffusion assay, A. foliicola and F. verticillioides inhibited the growth of M. morganii as a histamine-producing bacteria, and inhibiting histamine formation in fish. The best effects in inhibiting growth of the histamine-producing bacteria and histamine formation inhibition in fish were produced with F. verticillioides water fraction at 0°C incubation.
Endophytic Fungi from Four Indonesian Medicinal Plants and Their Inhibitory Effect on Plant Pathogenic Fusarium oxysporum Nampiah Sukarno; Rohani Cinta Badia Ginting; Utut Widyastuti; Latifah Kosim Darusman; Sihegiko Kanaya; Irmanida Batubara; I Nyoman Pugeg Aryantha; Mashuri Waite
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.152

Abstract

The medicinal plants Centella asiatica, Curcuma xanthorrhiza, Guazuma ulmifolia, and Hydrocotyle verticillata are widely used in Indonesian traditional medicine, but little is known about their associated endophytic fungi. This research aimed to study the diversity of endophytic fungi derived from functional parts of these plants and to evaluate their potential as antifungal agents against the plant pathogenic fungus Fusarium oxysporum. A total of 17 isolates of endophytic fungi were obtained: nine from leaves of G. ulmifolia, three each from leaves of C. asiatica and H. verticillata, and two from rhizomes of C. xanthorrhiza. The genus Colletotrichum was found in all plants studied, but each plant was associated with different species. Colletotrichum aeschynomenes was associated with C. xanthorrhiza, C. siamense was associated with C. asiatica, and C. tropicale was associated with G. ulmifolia and H. verticillata. The species Curvularia affinis, Diaporthe tectonae, Lasiodiplodia mahajangana, Parengyodontium album, Talaromyces trachyspermus, and Speiropsis pedatospora were found only in G. ulmifolia; while Didymella coffeae-arabicae and Muyocopron laterale were found only in H. verticillata. The endophytic fungi showed inhibition activity against F. oxysporum with inhibition values of 6.0-78.9%, T. trachyspermus JBd10 and C. affinis JBd14 gave the highest inhibition activity.
In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent Wendi Nurul Fadillah; Nampiah Sukarno; Dyah Iswantini; Min Rahminiwati; Novriyandi Hanif; Mashuri Waite
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.76-86

Abstract

This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.
Pemacuan Pertumbuhan Melon (Cucumis melo L.) dengan Cendawan Mikoriza Arbuskula dan Bakteri Azospirillum sp. Lady Diana Tetelepta; Triadiati ,; dan Nampiah Sukarno
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 2 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.165 KB) | DOI: 10.24831/jai.v44i2.13490

Abstract

ABSTRACTMelon (Cucumis melo L.) is a high economic value horticultural crop that is cultivated in some regions of Indonesia under fertilization management. Application of inorganic fertilizer continuously can reduce soil microbial abundance. One of the soil microbial that promote plant growth is arbuscular mycorrhizal fungi (AMF) and Azospirillum sp. The aim of this study was to analysed the effect of AMF and Azospirillum sp. in promoting growth and production of melon. The experiment was carried out in a greenhouse and was arranged in a completely randomized design with five replicates. Five treatments tested were: control, fertilized with NPK, inoculated with AMF, inoculated with Azospirillum sp., inoculated with AMF + Azospirillum sp. The results showed that the effect of AMF on root growth and shoot growth were similar to NPK fertilizer. Azospirillum sp. increased root growth. On the other side, the effect of Azospirillum sp. on shoot growth was similar to NPK fertilizer. However, AMF and Azospirillum sp. inoculation solely increased plant height, fruit weight, fruit diameter, flavor and length of fruit storage. Meanwhile, combination of AMF and Azospirillum sp. increased plant height, root growth, shoot growth, fruit weight, fruit diameter, flavor and length of fruit storage. This study revealed that application of AMF and Azospirillum sp. in melon cultivation was more effective and efficient than NPK fertilizer.Keywords: arbuscular mycorrhizal fungi, Azospirillum sp., Cucumis melo L.
Karakteristik Mikoriza Arbuskula Tanaman Serai Wangi (Cymbopogon nardus L.) di Lapangan Ternaungi dan Tidak Ternaungi Nampiah Sukarno; Rahayu Laelandi; Ibnu Qayim; Mega Putri Amelya
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.109

Abstract

Arbuscular mycorrhiza (AM) characteristics of citronella grass in the field have not been reported. This research aimed to study the AM characteristics of citronella grass grown in unshaded and shaded fields. The roots of citronella grass were collected from citronella grass plantations in Cianjur, West Java. The root samples were analyzed for AM structures, namely entry points, intercellular hyphae, arbuscules, and vesicles. The results showed that the citronella grass form AM colonization. The quality of root colonization differed between the two cultivation systems. The unshaded citronella grass had higher root colonization compared to shaded citronella grass. In the unshaded citronella grass, the number of arbuscules was 7 per cm of root length, whereas in the shaded citronella grass was 4 per cm of root length. The types of arbuscules observed were arum and intermediate. There were no differences in the number of entry points in the two cultivated systems, which was 3,5 entry points per cm of root length. The numbers of vesicles and internal hyphae in unshaded citronella grass were lower than that of in the shaded citronella grass. In the unshaded citronella grass, the number of vesicles and intracellular hyphae were 1,5 and 8,5 per cm root length, whereas in the shaded citronella grass were 3,5 and 11 per cm root length, respectively. Shading plants grown in the field were bamboo, banana, coffee, tea, and sugar palm. All the shading plants formed AM symbiosis with a colonization value of 7 to 30%. This research indicates that arbuscular mycorrhiza is an important component in the citronella grass cultivation in unshaded and shaded fields. Keywords: Arbuscule, entry point, intercellular hyphae, root colonization, vesicle
Co-Authors , Triadiati ADI YULANDI Agustin Wydia Gunawan Alex Hartana ALEX HARTANA Alex Hartana Anastastia Raditya Hidayanti Astri Ariyani Atit Kanti Atit Kanti Cici Rahmawati Cinta Badia Br Ginting, Rohani Dedi Fardiaz Desi Maria Panjaitan Didiek Hadjar Goenadi Dyah Iswantini Edhi Sandra Edi Guhardja Endang Sukara Eris Septiana Fachrian Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadillah, Wendi Nurul Franco, Christopher MM Hamim Hamim HAPPY WIDIASTUTI Hidayanti, Anastastia Raditya I Made Sudiana I Nyoman Pugeg Aryantha Ibnu Qayim Irmanida Batubara Kartini Kramadibrata Kemala S. Nagur L K Darusman Lady Diana Tetelepta Latifah K Darusman latifah K Darusman Latifah Kosim Darusman LATIFAH KOSIM DARUSMAN Latifah Kosim Darusman M. Rafi Mashuri Waite Mashuri Waite Maulani, Nail Izzatul Mega Putri Amelya Michael Michael Mien Ahmad Rifai Mujahidah, Shofia Mujahidah, Shofia Mulya Sari Nadia T. Hendartina Nadiya Dwi Rahayu Novriyandi Hanif Partomuan Simanjuntak Putra, Sukma Triperdana Rahayu Laelandi Rahayu, Nadiya Dwi Rahminiwati, Min Rida Oktorida Khastini Risna Riza Firmansyah Riza Zainuddin Ahmad Rohani Cinta Badia Ginting ROHANI CINTA BADIA GINTING Sally SMITH SIHEGIKO KANAYA Sihegiko Kanaya Sintho Wahyuning Ardie Soekisman Tjitrosoedirdjo Sofi Mursidawati Sofi Mursidawati Sri Listiyowati Subagya, Mahmud Sukarno Sukarno Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulfiah, Siti Syaiful Anwar Utut Widyastuti UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Suharsono Utut Widyastuti Suharsono† Waite, Mashuri Wei Zhang Wendi Nurul Fadillah Wendi Nurul Fadillah Wendi Nurul Fadillah Yanti Novera Yasuyuki Hashidoko† Yuyun Nisaul Khairillah Za’aziza Ridha Julia