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All Journal ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education
Yopi Yopi
Research Center for Biotechnology, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Glucoamylase Production by Aspergillus awamori KT-11 In Solid State Fermentation Using Cassava Peel as Substrate Urip Perwitasari; Nuryati Nuryati; Ruth Melliawati; Yopi Yopi
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.187 KB) | DOI: 10.14203/ann.bogor.2017.v21.n1.21-28

Abstract

In order to utilization of cassava peel waste this study tries to produce glucoamylase by solid state fermentation with Aspergillus awamori KT-11. Composition medium and drying technique are affecting the glucoamylase production. The highest glucoamylase activities were from cassava peel plus mineral medium. Activity glucomaylase in cassava peel plus mineral medium by oven drying was 365 U/mL and freeze dring was 452 U/mL.  It is conclud cassava peel plus mineral is a better substrate for glucoamylase production from A. awamori KT-11 in solid state fermentation. Powder of glucoamylase also proved capable of hydrolyzing starch-based biomass. 
Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus Awan Purnawan; Yopi Yopi; Tun Tedja Irawadi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.9201

Abstract

The increase of public attention to health has prompted researchers to look for new sources of functional food. Palm Cake Kernel (PKC) waste was abundant in Indonesia, Oligosaccharide has an important benefit for human health. Recently oligosaccharide is not only important as an artificial sweetener, but also as a functional food component. This study was aimed to produce oligo-mannan enzymatically from PKC waste using mannanase derived from of Streptomyces cyaenus isolates of indigenous Indonesia. The enzyme concentration was determined by enzyme activity assay while oligo-mannan content in the PKC was analyzed using TLC and HPLC. Mannanase enzyme activity of 1706 U/ml on the second day of agitation 200 rpm at a temperature of 30°C Hydrolysis of mannooligomannan by using mannanase produced by streptomyces cyaenus. The optimum mannanase enzyme activity obtained on day 2 with the value of the activity as much of 0.702 U/mL. The protein content of the 2nd day at an agitation speed of 150 rpm, 200 rpm, and 250 rpm, respectively, were 1783, 1950 and 2283 ppm. Streptomyces cyaenus is Indonesian original isolates potentially producing mannanase that can produce mannooligomannan.
Co-Authors Awan Purnawan Nuryati Nuryati Ruth Melliawati Tun Tedja Irawadi Urip Perwitasari