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All Journal OLDI (Oseanologi dan Limnologi di Indonesia) Marine Research in Indonesia
Lies Indah Sutiknowati
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science

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Isolasi dan Identifikasi Bakteri Toleran Logam Berat dari Sedimen Mangrove di Pengudang dan Tanjung Uban, Pulau Bintan, Indonesia de Fretes, Charlie Ester; Sutiknowati, Lies Indah; Falahudin, Dede
OLDI (Oseanologi dan Limnologi di Indonesia) Vol 4, No 2 (2019)
Publisher : Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.267 KB) | DOI: 10.14203/oldi.2019.v4i2.244

Abstract

Isolation and Identification of Heavy Metals Tolerant Bacteria from Mangrove Sediment in Pengudang and Tanjung Uban, Bintan Island, Indonesia. Bacterial searches for remediation of heavy metal pollutants continue to be carried out due to the increasing pollution in the ecosystem as a result of industrialization. This research aims to identification bacteria strains that are tolerant of heavy metals from mangrove sediments in the Pengudang and Tanjung Uban, Bintan Island in 2018. Bacterial isolation was carried out by pour plate method and purified using marine agar (MA) medium. Screening of bacterial isolates was carried out by growing isolates on MA medium with the addition of 100 ppm Pb. In addition, the determination of minimum inhibitory concentration (MIC) was performed on the isolates were tolerant to Pb, Cu, and Cd by addition of heavy metals in 50-1000 ppm concentrations. Identification of heavy metal tolerant isolates was carried out by sequencing the 16S rRNA gene. The results of bacterial isolation from mangrove sediments obtained 48 isolates used for testing the ability of heavy metals tolerant. Screening tests showed that PGD1A, PGD5A, PGD22A, PGD1B, PGD9B, PGD5C, PGD6C, TJU1, TJU5, and TJU7 isolates were heavy metal resistant. The results showed that bacterial isolated from mangrove sediments had MIC values of 800-900 ppm for Pb, 100-800 ppm for Cu and 100-200 ppm for Cd. There are 3 isolates that have high resistance to three kinds of heavy metals such as Bacillus oceanisediminis PGD1A, Vibrio alginolyticus PGD5A, and Halobacillus kuroshimensis PGD9B. This indicates that the isolate can be used efficiently for removal of heavy metals from the ecosystem.
BIOREMEDIATION STUDY: HYDROCARBON DEGRADING BACTERIA Sutiknowati, Lies Indah
Marine Research in Indonesia Vol 32 No 2 (2007)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/mri.v32i2.442

Abstract

Many microorganisms capable of degrading petroleum components have been isolated and few of them seem to be important for petroleum biodegradation in natural environments. To identify the bacteria that play a major role in degradation of petroleum polynuclear aromatic hydrocarbons (PAHs), bacteria were enriched from seawater by using Naphthalene, Phenanthrene, Trichlorodibenzofuran and Benzo[a]pyrene as a carbon and energy source. The result of study that members of the genus Alcanivorax and Thalassospira became predominant in the enrichment cultures. The strains isolated in this study could grow on crude oil and degraded PAH components of crude oil. The number of cells increased to 8.1x106 cells g-1 after 14 days in subculture. PAH degradation proceeded parallel with the growth of bacteria cells. This observation which has been conducted in Marine Biotechnology Institute, Kamaishi, Iwate-ken, Japan suggests that Alcanivorax and Thalassospira play an important role in the degradation of petroleum PAHs in marine environment.
Co-Authors Charlie Ester de Fretes Dede Falahudin