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Eva Marliana
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science

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ISOLATION AND CHARACTERIZATION OF FLAVONOID COMPOUNDS FROM ETHYL ACETATE FRACTION OF Macaranga hosei LEAVES Frenki Alan Nuari; Eva Marliana; Daniel Daniel
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Isolation and characterization of flavonoid compounds in the third fraction of ethyl acetate of Macaranga hosei leaves. The separation process in the third fraction of ethyl acetate was perfomed using gravity column chromatography with gradient elution method. The obtained isolate was tested for its purity using thin layer chromatography with various eluents named n-hexane, chloroform, acetone, ethyl acetate and methanol with Rf values 0; 0,17; 0,86; 0,97 and 0,81 respectively. The isolate was characterized as MH-1 phenolic compound as much as 30 mg in the form of oil. Analysis of MH-1 compound with UV showed 2 peaks at ? 295 nm (band II) and 340 nm (band I). Analysis of MH-1 compounds with FT-IR showed OH functional groups, aliphatic C-H, aromatic C-H, C=O, aromatic C=C, C-O-C and C-OH. Based on the results of the UV and FT-IR spectra, the MH-1 compound is assumed to be of flavonoids.
SCREENING BACTERIAL OF ENDOPHYTIC PRODUCING AMYLASE, LIPASE AND PROTEASE FROM LEAVES OF Macaranga hullettii King ex Hook.f. Sherly Pricilia; Winni Astuti; Eva Marliana
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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The objective of this study was to screening test of endophytic bacteria producing amylase, lipase and protease from leaves Macaranga hullettii King ex Hook.f. Endophytic bacteria isolation was performed on nutrient agar (NA) and 21 single colonies of endophytic bacteria were obtained. Endophytic bacteria that all of the endophytic bacteria positive producing enzym, 8 of the endophytic bacteria positive amylase, 7 of the endophytic bacteria positive lipase and 4 of the endophytic bacteria positive protease.
ISOLATION AND CHARACTERIZATION OF FLAVONOID COMPOUNDS FROM ETHYL ACETATE FRACTION OF Macaranga lamellata Whitmore LEAVES Sherly Indah Puspita; Eva Marliana; Daniel Daniel
JURNAL ATOMIK Vol 4 No 1 (2019)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.924 KB)

Abstract

Isolation and characterization of flavonoid compounds from ethyl acetate fraction of Macaranga lamellata Whitmore leaves have been done in this research. Macaranga lamellata Whitmore leaves was macerated using methanol and fractionated with n-hexane and ethyl acetate. The separation process of ethyl acetate fraction was conducted using gravity column chromatography by Step Gradient Polarity (SGP) method. The isolate was tested for its purity using thin layer chromatography with various eluents i.e. n-hexane, chloroform, methanol, acetone and ethyl acetate, with Rf values were 0; 0,7857; 0,8333; 0,9048 and 0,9286 respectively. The result indicated that the isolate was obtained as ML1 (3 mg) belong to flavonoid compound in the form of oil. Analysis of ML1 compound using UV displayed 2 peaks at ? 291 nm (band II) and 342 nm (band I). Analysis of ML1 compound using FT-IR displayed functional groups -OH, C-H aromatic, C-H aliphatic, C=C aromatic, C=O, C-OH and C-O-C. Based on the results of UV and FT-IR spectra, ML1 compound was suspected to contain flavonoid compound.
Co-Authors Daniel Daniel Frenki Alan Nuari Sherly Indah Puspita Sherly Pricilia Winni Astuti