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All Journal SAINSMAT Journal of Tropical Soils Indonesian Journal of Cancer Chemoprevention
. Syafruddin
Universitas Hasanuddin Eijkman Institute of Molecular Biology
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology

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Isolation and Structure Elucidation of Soulatro Coumarin From Stem Bark of Calophyllum soulattri Burm F and In Vivo Antiplasmodial Activity by Using Mice Infected by Plasmodium berghei Abbas, Jamilah; Darmawan, Achmad; Syafruddin, .
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

The soulatro coumarin compound was isolated and elucidated from the stem bark of Calophyllum soulattri Burm F, the samples were collected from Jayapura Papua Irian Island in  Indonesia.  Isolation  process  was  done  by  maceration  at  room  temperature  in  methanol, than  partitioned  in  a  mixture  of  n  hexane-water  (1:1),  followed  by  dichloromethane-water (1:1)    and  ethyl  acetate-water  (1:1).  A  portion  of  ethyl  acetate  extract  was  subjected  to column chromatography over silica gel packed and eluted with n-hexane a gradient of ethyl acetate to 100% followed by CHCl3  in MeOH (20:1, 10 :1, 5:1, 1:1). Fraction  B (CHCl3 in MeOH 20:1) was subjected to column chromatography  over silica gel 300 mesh  and eluted with  EtOAc-MeOH  mixtures  of  increasing  polarity.  Faction  with  the  same  Rf  valeus  were combined  and  eluted  with  EtOAc-MeOH    (19:1)  showed  one  spot  on  TLC.  They  were combined  and  evaporated  to  yield  a  solid  than  was  recrystallized  in  mixture  of  CH2Cl2-methanol  to  give  soulatro  coumarin  compound.  The  structure  was  determinated  by spectroscopic analysis, in particular by 1D and 2D NMR techniques, from these spectra data conclution  that  compound  is  soulatro  coumarin.  Antimalarial  assay  was  tested  against Plasmodium berghei parasite as in vivo using 18 mices rodent wich was infected by  Plasmodium berghei  parasite.  The  soulatro  coumarin    showed  activity  against  P.  berghei  with  dosage 0.0005867  mM/1  kg  body  weight  ;  0.005867  mM/1  kg  bw;  0.05867  mM/1  kg  bw;  0.5867 mM/1  kg  bw  5.867  mM/1  kg  bw  and  58.67  mM/1  kg  bw  could  inhibite  growth  rate  of parasite = 57.32%; 63.37%; 43.02%; 53.49%; 47.67% respectively.   Keywords : Antiplasmodial activity, coumarin, Calophyllum soulattri Burm. F, in vivo,  Chloroquine, Plasmodium berghei.
Analisis Mutasi Gen Pfmdr1 1246 pada Penderita Malaria di Mamuju Sulawesi Barat Yusuf, Yenny; Hartono, .; Syafruddin, .
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 2, No 2 (2013): September
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.467 KB) | DOI: 10.35580/sainsmat228572013

Abstract

Parasit Plasmodium falciparum dapat mengembangkan mekanisme resistensi terhadap obat anti-malaria melalui mutasi gen yang terjadi pada parasit tersebut, termasuk gen Plasmodium falciparum multi-drug resistance protein 1 (Pfmdr1). Mutasi pada gen tersebut dapat terjadi pada beberapa posisi codon, termasuk posisi 1246, yaitu perubahan asam aspartat ke tirosin. Alel mutan 1246Y telah dikaitkan dengan kegagalan terapi anti-malaria amodiaquine. Penelitian ini dilakukan untuk menganalisis mutasi gen Pfmdr1 1246 pada penderita malaria di kabupaten Mamuju provinsi Sulawesi Barat yang akan memulai terapi kombinasi artesunate-amodiaquin (AS-AQ). Dari 18 sampel penderita malaria, analisis gen tersebut menunjukkan tidak ada alel 1246Y pada seluruh sampel. Hasil follow-up pasien selama 28 hari menunjukkan regimen AS-AQ efektif sebagai terapi anti-malaria di daerah tersebut.Kata kunci: Pfmdr1, 1246Y, Resistensi Obat Anti-Malaria, Artesunate-Amodiaquin
Rekomendasi Pemupukan P Untuk Tanaman Jagung Pada Tanah Inceptisols Menggunakan Pendekatan Uji Tanah Syafruddin, .
JOURNAL OF TROPICAL SOILS Vol. 13 No. 2: May 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i2.95-102

Abstract

Productivity of maize in Central Sulawesi has been significantly low. The evidence has been a challenge on maize development in this area. Rational fertilization based on soil conditions and nutrient requirement of plants croped, has saved use of fertilizer, increased efficiency of the farm and also reduced negative impacts of chemical fertilizer. This will sustain the use of land resource. The researceh aimed to establish phosphorus fertilizer recomendation for maize crops at several levels of soil nutrients status in dryland rationally and spesicific location. The research was conducted in for stages i.e.: (1) survey on nutrient status, (2) calibration test and correlation study, (3) availability class determination of P and (4) composting P fertilizer recomendation. Split plot design was used. Main plot was soil nutrients status, consisting of 4 levels and sub plot was fertilizer dosage consisting of 5 levels, with 3 replications. The size of each plot was 4 m x 5 m. The results showed that the treatments applied indicated an interaction. Nutrient availability class and plant responses were divided by tree classes i.e: low, medium and high level with extraction methods (reagents) that excellently correlate, are Trough, Colwell and Bray 1 methods. Optimun dosage of P fertilizer for treatment were,  76 kg P2 O5 ha -1 for  low nutrient status 41 kg P2 O5  ha -1 for medium  nutrient status and   high nutrient status to nutrient status very high, P fertilizer was not required.
Co-Authors Achmad Darmawan Hartono Jamilah Abbas Yenny Yusuf