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All Journal Maspari Journal Marine Research in Indonesia Makara Journal of Science
Hikmah Thoha
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Earth & Planetary Sciences

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IDENTIFICATION OF SOME STRAINS OF DINOFLAGELLATES BASED ON MORPHOLOGY AND MOLECULAR ANALYSIS Thoha, Hikmah
Makara Journal of Science Vol. 12, No. 2
Publisher : UI Scholars Hub

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Abstract

Dinoflagellates are the important primary producers in aquatic environments. In oceans, they play interesting role in ecological functions such as red tide forming organisms, symbiont of coral reef or sea anemone and DSP (Diarrhetic Shellfish Poisoning) or PSP (Paralytic Shellfish Poisoning) producing organisms. Morphology and molecular analysis of dinoflagellates were conducted on November 2002 to March 2003. The phylogenetic studies based on 18S rDNA analyses, sequence have begun to appear more frequently in the literature, as attention has turned to relationships within the major eukaryotic lineages, particular importance for the taxonomy of the armored and unarmored genera of dinoflagellates (Gyrodinium sp., Cachonina sp., Gymnodinium sp., Amphidinium sp.), because many of the genera cause extensive plankton blooms, fish kills and other harmful events, were studied used to amplify 18S rDNA, present in the total DNA extracted from algal pellet. The amplify approximately 1400 bp of the nuclear-encoded LSU rDNA gene using terminal primeirs DIR, products were cheked by 1.0 % agarose gel electrophoresis, then cloning with TA cloning KIT. Sequencing were analyzed by the GENETIX Mac Software, Homology search by Blast and Phylogenetic analysis. Results of hylogenetic analysis of 18S rDNA are: Strain no. 10893 (un identified) from the genera, it is belonging Gymnodinium or Polarella. Strain no. 10795 is closely related other species Cachonina hallii. We tentatively named strain no 11151 and 11160 similar to Gyrodinium or Gymnodinium based on morphology, but these strain indepently position in this tree and is not a real of Gymnodinium sensu stricto. It is possible, we can establish the new genera for strain no. 11151; 11160 because this not cluster any other unarmored species.
IDENTIFICATION OF SOME STRAINS OF DINOFLAGELLATES BASED ON MORPHOLOGY AND MOLECULAR ANALYSIS Thoha, Hikmah
Makara Journal of Science Vol. 11, No. 2
Publisher : UI Scholars Hub

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Abstract

Dinoflagellates are the important primary producers in aquatic environments. In oceans, they play interesting role in ecological functions such as red tide forming organisms, symbiont of coral reef or sea anemone and DSP (Diarrhetic Shellfish Poisoning) or PSP (Paralytic Shellfish Poisoning) producing organisms. Morphology and molecular analysis of dinoflagellates were conducted on November 2002 to March 2003. The phylogenetic studies based on 18S rDNA analyses, sequence have begun to appear more frequently in the literature, as attention has turned to relationships within the major eukaryotic lineages, particular importance for the taxonomy of the armored and unarmored genera of dinoflagellates (Gyrodinium sp., Cachonina sp., Gymnodinium sp., Amphidinium sp.), because many of the genera cause extensive plankton blooms, fish kills and other harmful events, were studied used to amplify 18S rDNA, present in the total DNA extracted from algal pellet. The amplify approximately 1400 bp of the nuclear-encoded LSU rDNA gene using terminal primeirs DIR, products were cheked by 1.0 % agarose gel electrophoresis, then cloning with TA cloning KIT. Sequencing were analyzed by the GENETIX Mac Software, Homology search by Blast and Phylogenetic analysis. Results of hylogenetic analysis of 18S rDNA are: Strain no. 10893 (un identified) from the genera, it is belonging Gymnodinium or Polarella. Strain no. 10795 is closely related other species Cachonina hallii. We tentatively named strain no 11151 and 11160 similar to Gyrodinium or Gymnodinium based on morphology, but these strain indepently position in this tree and is not a real of Gymnodinium sensu stricto. It is possible, we can establish the new genera for strain no. 11151; 11160 because this not cluster any other unarmored species.
KELIMPAHAN PLANKTON DI EKOSISTEM PERAIRAN TELUK GILIMANUK, TAMAN NASIONAL, BALI BARAT Thoha, Hikmah
Makara Journal of Science Vol. 11, No. 1
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Abstract

Plankton abundance in Gilimanuk Bay of National Park Ecosystem, West Bali. An observation of plankton condition in Gilimanuk Bay of National Park, West Bali was conducted during March 2006. This study aimed to observ the environmental quality of Gilimanuk Bay water. The parameters observed were focused on the phytoplankton and zooplankton communities. Ten points of observation was done. Plankton abundance varied with location group from 4428 to 1716224 sel/m3 and 23938 individu/m3 (67.73 %) for microplankton and macroplankton, respectively. Microplankton community structure was dominated by the group of diatoms, such as Coscinodiscus, Chaetoceros, Guinardia, Navicula. Pseudonitzshia. The genus Ceratium (the group of dinoflagellates) was found in relatively abundant, but still normal condition. The structure of macroplankton was dominated by copepods 23938 individu/m3 (67.73 %). The other hand, information about mangrove, sea grass and coral reef and asssosiation with fauna in these ecosystem of Gilimanuk Bay very rarely. We need observed this subject for base line data to improving management of marine resources development.
PENGARUH MUSIM TERHADAP PLANKTON DI PERAIRAN RIAU KEPULAUAN DAN SEKITARNYA Thoha, Hikmah
Makara Journal of Science Vol. 7, No. 2
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Abstract

Seasonal influenzed on plankton of the waters around Riau islands. The observation was conducted during April - May 2002. This study was done in relation with observation on environment quality of the waters around Riau Islands. The parameter observed were focus on the plankton communities for tweentyfives points of stations which were grouped into two zone based on the distance of the each station. Plankton abundance varied with location groups from 50- 90 % respectively. Community structure was dominated by the group of diatoms such as Chaetoceros, Dytilum, Nitzschia, Thalassionema, Thalassiothrix and the genus Ceratium (the group of Dinoflagellata). Community structure of zooplankton was dominated by the group of Copepods (45 – 90 %) and Polychaeta, Chaetognata, Bivalvia, Gastropods and Oikopleura (more than 90 %). The results describe the relationship between the water environmental condition and variation of plankton condition
IDENTIFICATION OF SOME STRAINS OF DINOFLAGELLATES BASED ON MORPHOLOGY AND MOLECULAR ANALYSIS Thoha, Hikmah
Makara Journal of Science Vol. 12, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dinoflagellates are the important primary producers in aquatic environments. In oceans, they play interesting role in ecological functions such as red tide forming organisms, symbiont of coral reef or sea anemone and DSP (Diarrhetic Shellfish Poisoning) or PSP (Paralytic Shellfish Poisoning) producing organisms. Morphology and molecular analysis of dinoflagellates were conducted on November 2002 to March 2003. The phylogenetic studies based on 18S rDNA analyses, sequence have begun to appear more frequently in the literature, as attention has turned to relationships within the major eukaryotic lineages, particular importance for the taxonomy of the armored and unarmored genera of dinoflagellates (Gyrodinium sp., Cachonina sp., Gymnodinium sp., Amphidinium sp.), because many of the genera cause extensive plankton blooms, fish kills and other harmful events, were studied used to amplify 18S rDNA, present in the total DNA extracted from algal pellet. The amplify approximately 1400 bp of the nuclear-encoded LSU rDNA gene using terminal primeirs DIR, products were cheked by 1.0 % agarose gel electrophoresis, then cloning with TA cloning KIT. Sequencing were analyzed by the GENETIX Mac Software, Homology search by Blast and Phylogenetic analysis. Results of hylogenetic analysis of 18S rDNA are: Strain no. 10893 (un identified) from the genera, it is belonging Gymnodinium or Polarella. Strain no. 10795 is closely related other species Cachonina hallii. We tentatively named strain no 11151 and 11160 similar to Gyrodinium or Gymnodinium based on morphology, but these strain indepently position in this tree and is not a real of Gymnodinium sensu stricto. It is possible, we can establish the new genera for strain no. 11151; 11160 because this not cluster any other unarmored species.
Co-Authors Aditya, Vishnu Arief Rachman M. Yogi Riyantama Isjoni Nurul Fitriya Riris Aryawati Tumpak Sidabutar